Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,497,707	T→C	L21P (CTG→CCG)	gldA →	glycerol dehydrogenase, NAD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,497,707	0	T	C	100.0%	96.3 / NA	29	L21P (CTG→CCG)	gldA	glycerol dehydrogenase, NAD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/17);  total (12/17)

TACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTA  >  W3110S.gb/3497676‑3497744                                |                                      tACATCCAGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCg                    <  1:507661/51‑1 (MQ=255) tACATCCAGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCg                    <  1:218078/51‑1 (MQ=255) tACATCCAGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCg                    <  1:2037680/51‑1 (MQ=255) tACATCCAGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCg                    <  1:2382003/51‑1 (MQ=255) tACATCCAGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCg                    <  1:2493974/51‑1 (MQ=255) tACATCCAGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCg                    <  1:2567391/51‑1 (MQ=255) tACATCCAGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCg                    <  1:1729005/51‑1 (MQ=255) tACATCCAGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCg                    <  1:485055/51‑1 (MQ=255) tACATCCAGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCg                    <  1:155915/51‑1 (MQ=255) tACATCCAGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCg                    <  1:1541200/51‑1 (MQ=255) tACATCCAGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCg                    <  1:551435/51‑1 (MQ=255) tACATCCAGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCg                    <  1:827156/51‑1 (MQ=255) tACATCCAGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCg                    <  1:1273617/51‑1 (MQ=255) tACATCCAGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCg                    <  1:1173223/51‑1 (MQ=255) tACATCCAGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCg                    <  1:1238552/51‑1 (MQ=255)  aCATCCAGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCg                    <  1:1335678/50‑1 (MQ=255)  aCATCCAGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCg                    <  1:1988658/50‑1 (MQ=255)        aGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCGCTGGCAGaa           >  1:963284/1‑53 (MQ=255)        aGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTa  >  1:2009061/1‑62 (MQ=255)        aGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTa  >  1:1998437/1‑62 (MQ=255)        aGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTa  >  1:1949495/1‑62 (MQ=255)        aGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTa  >  1:483177/1‑62 (MQ=255)        aGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTa  >  1:1621414/1‑62 (MQ=255)        aGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTa  >  1:1564447/1‑62 (MQ=255)        aGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTa  >  1:146834/1‑62 (MQ=255)        aGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTa  >  1:801570/1‑62 (MQ=255)        aGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTa  >  1:1273678/1‑62 (MQ=255)        aGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTa  >  1:959063/1‑62 (MQ=255)        aGGGCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTa  >  1:123931/1‑62 (MQ=255)                                |                                      TACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTA  >  W3110S.gb/3497676‑3497744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑