Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,521,418	T→C	V106A (GTA→GCA)	glpX →	fructose 1,6‑bisphosphatase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,521,418	0	T	C	100.0%	295.2 / NA	86	V106A (GTA→GCA)	glpX	fructose 1,6‑bisphosphatase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/81);  total (5/81)

CGCATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGTAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGTAT  >  W3110S.gb/3521372‑3521458                                               |                                         cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:373736/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:2096748/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:2273272/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:2282114/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:2326546/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:236294/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:2377602/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:246702/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:2543820/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:2554627/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:2608757/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:2668760/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:2773400/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:2861836/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:1085111/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:398573/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:450957/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:490269/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:526302/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:536425/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:538537/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:637386/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:687589/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:748458/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:807708/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:826129/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:831254/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:946153/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:1964960/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:1347934/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:1478479/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:1484926/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:1485597/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:1177755/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:1162760/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:114833/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:1103246/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:1647399/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:1692096/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:2082525/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:190524/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:1748854/62‑1 (MQ=255) cgcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:1853665/62‑1 (MQ=255)  gcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:2380962/61‑1 (MQ=255)  gcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:559531/61‑1 (MQ=255)  gcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:2408881/61‑1 (MQ=255)  gcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:1480019/61‑1 (MQ=255)  gcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:2370479/61‑1 (MQ=255)  gcATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:671247/61‑1 (MQ=255)    aTGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:2310791/59‑1 (MQ=255)        cGGCGATGGGCCAGGCTTACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:2363444/55‑1 (MQ=255)            gATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:2379733/51‑1 (MQ=255)            gATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGgctg                           <  1:2125841/51‑1 (MQ=255)              tGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTc                       >  1:94064/1‑53 (MQ=255)              tGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATgcg              >  1:1740174/1‑62 (MQ=255)              tGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATgcg              >  1:1643005/1‑62 (MQ=255)              tGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATgcg              >  1:90735/1‑62 (MQ=255)              tGGGCCAGGCTAACGCGCTGGCGGTGCTGGAAGCAGGCGATAAAGGCTGCTTCCTAAATgcg              >  1:308069/1‑62 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:979934/60‑1 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:549898/60‑1 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:101554/60‑1 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:614551/60‑1 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:1280010/60‑1 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:1253278/60‑1 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:69147/60‑1 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:697926/60‑1 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:950051/60‑1 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:867900/60‑1 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:1538885/60‑1 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:1916907/60‑1 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:1874160/60‑1 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:2506469/60‑1 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:2521144/60‑1 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:1616222/60‑1 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:2694609/60‑1 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:272975/60‑1 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:537861/60‑1 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:2804873/60‑1 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:1534622/60‑1 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:286420/60‑1 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:1497343/60‑1 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:424783/60‑1 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:443734/60‑1 (MQ=255)                            gcgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:1371583/60‑1 (MQ=255)                             cgcTGGCGGTGCTGGCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:2386338/59‑1 (MQ=255)                                          ggCAGCAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGtat  <  1:37110/46‑1 (MQ=255)                                               |                                         CGCATGACGGCGATGGGCCAGGCTAACGCGCTGGCGGTGCTGGCAGTAGGCGATAAAGGCTGCTTCCTCAATGCGCCGGATATGTAT  >  W3110S.gb/3521372‑3521458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑