Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,536,648	A→G	K165E (AAA→GAA)	rhaT →	L‑rhamnose:proton symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,536,648	0	A	G	95.8%	74.8 / ‑6.2	24	K165E (AAA→GAA)	rhaT	L‑rhamnose:proton symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (8/15);  minor base T (0/1);  total (8/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCCGGGCAGTTGAAAGAGCGCAAGATGGGCATTAAAGCCGAAGAGTTCAATCTGAAAAAAGGGCTGGTGC  >  W3110S.gb/3536613‑3536684                                    |                                     gcgcCGGGCAGTTGAAAGAGCGCAAGATGGGCATTGAAGCCGAAGAGTTCAATCTGaaaaaa            >  1:133616/1‑62 (MQ=255) gcgcCGGGCAGTTGAAAGAGCGCAAGATGGGCATTGAAGCCGAAGAGTTCAATCTGaaaaaa            >  1:976220/1‑62 (MQ=255) gcgcCGGGCAGTTGAAAGAGCGCAAGATGGGCATTGAAGCCGAAGAGTTCAATCTGaaaaaa            >  1:772832/1‑62 (MQ=255) gcgcCGGGCAGTTGAAAGAGCGCAAGATGGGCATTGAAGCCGAAGAGTTCAATCTGaaaaaa            >  1:398387/1‑62 (MQ=255) gcgcCGGGCAGTTGAAAGAGCGCAAGATGGGCATTGAAGCCGAAGAGTTCAATCTGaaaaaa            >  1:2710654/1‑62 (MQ=255) gcgcCGGGCAGTTGAAAGAGCGCAAGATGGGCATTGAAGCCGAAGAGTTCAATCTGaaaaaa            >  1:2458623/1‑62 (MQ=255) gcgcCGGGCAGTTGAAAGAGCGCAAGATGGGCATTGAAGCCGAAGAGTTCAATCTGaaaaaa            >  1:2054422/1‑62 (MQ=255) gcgcCGGGCAGTTGAAAGAGCGCAAGATGGGCATTGAAGCCGAAGAGTTCAATCTGaaaaaa            >  1:1095814/1‑62 (MQ=255)           gTTGAAAGAGCGCAAGATGGGCATTGAAGCCGAAGAGTTCAATCTGAAAAAAGGGCTGGTGc  <  1:600400/62‑1 (MQ=255)           gTTGAAAGAGCGCAAGATGGGCATTGAAGCCGAAGAGTTCAATCTGAAAAAAGGGCTGGTGc  <  1:844334/62‑1 (MQ=255)           gTTGAAAGAGCGCAAGATGGGCATTGAAGCCGAAGAGTTCAATCTGAAAAAAGGGCTGGTGc  <  1:794838/62‑1 (MQ=255)           gTTGAAAGAGCGCAAGATGGGCATTGAAGCCGAAGAGTTCAATCTGAAAAAAGGGCTGGTGc  <  1:1406389/62‑1 (MQ=255)           gTTGAAAGAGCGCAAGATGGGCATTGAAGCCGAAGAGTTCAATCTGAAAAAAGGGCTGGTGc  <  1:769732/62‑1 (MQ=255)           gTTGAAAGAGCGCAAGATGGGCATTGAAGCCGAAGAGTTCAATCTGAAAAAAGGGCTGGTGc  <  1:766408/62‑1 (MQ=255)           gTTGAAAGAGCGCAAGATGGGCATTGAAGCCGAAGAGTTCAATCTGAAAAAAGGGCTGGTGc  <  1:732270/62‑1 (MQ=255)           gTTGAAAGAGCGCAAGATGGGCATTGAAGCCGAAGAGTTCAATCTGAAAAAAGGGCTGGTGc  <  1:621065/62‑1 (MQ=255)           gTTGAAAGAGCGCAAGATGGGCATTGAAGCCGAAGAGTTCAATCTGAAAAAAGGGCTGGTGc  <  1:2014651/62‑1 (MQ=255)           gTTGAAAGAGCGCAAGATGGGCATTGAAGCCGAAGAGTTCAATCTGAAAAAAGGGCTGGTGc  <  1:583787/62‑1 (MQ=255)           gTTGAAAGAGCGCAAGATGGGCATTGAAGCCGAAGAGTTCAATCTGAAAAAAGGGCTGGTGc  <  1:292842/62‑1 (MQ=255)           gTTGAAAGAGCGCAAGATGGGCATTGAAGCCGAAGAGTTCAATCTGAAAAAAGGGCTGGTGc  <  1:1498407/62‑1 (MQ=255)           gTTGAAAGAGCGCAAGATGGGCATTGAAGCCGAAGAGTTCAATCTGAAAAAAGGGCTGGTGc  <  1:2701934/62‑1 (MQ=255)           gTTGAAAGAGCGCAAGATGGGCATTGAAGCCGAAGAGTTCAATCTGAAAAAAGGGCTGGTGc  <  1:2682493/62‑1 (MQ=255)           gTTGAAAGAGCGCAAGATGGGCATTGAAGCCGAAGAGTTCAATCTGAAAAAAGGGCTGGTGc  <  1:1945524/62‑1 (MQ=255)                         aGATGGGGATTTAAGCCGAAGAGTTCAATCTGAAAAAAGGGCTGGTGc  <  1:1464228/48‑1 (MQ=39)                                    |                                     GCGCCGGGCAGTTGAAAGAGCGCAAGATGGGCATTAAAGCCGAAGAGTTCAATCTGAAAAAAGGGCTGGTGC  >  W3110S.gb/3536613‑3536684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑