| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,551,414 | A→G | M186V (ATG→GTG) | fdoG → | formate dehydrogenase‑O, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,551,414 | 0 | A | G | 100.0% | 18.1 / NA | 7 | M186V (ATG→GTG) | fdoG | formate dehydrogenase‑O, large subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (0/7); total (0/7) | |||||||||||
GGCTATTTAACGCAAAAATTCTCCCGCGCGCTGGGTATGCTCGCGGTCGACAACCAGGCGCG > W3110S.gb/3551378‑3551439 | ggCTATTTAACGCAAAAATTCTCCCGCGCGCTGGGTGTGCTCGCGGTCGACAACCAGGcgcg < 1:1054345/62‑1 (MQ=255)ggCTATTTAACGCAAAAATTCTCCCGCGCGCTGGGTGTGCTCGCGGTCGACAACCAGGcgcg < 1:1521023/62‑1 (MQ=255)ggCTATTTAACGCAAAAATTCTCCCGCGCGCTGGGTGTGCTCGCGGTCGACAACCAGGcgcg < 1:2726229/62‑1 (MQ=255)ggCTATTTAACGCAAAAATTCTCCCGCGCGCTGGGTGTGCTCGCGGTCGACAACCAGGcgcg < 1:595210/62‑1 (MQ=255)ggCTATTTAACGCAAAAATTCTCCCGCGCGCTGGGTGTGCTCGCGGTCGACAACCAGGcgcg < 1:667936/62‑1 (MQ=255)ggCTATTTAACGCAAAAATTCTCCCGCGCGCTGGGTGTGCTCGCGGTCGACAACCAGGcgcg < 1:885467/62‑1 (MQ=255) gCTATTTAACGCAAAAATTCTCCCGCGCGCTGGGTGTGCTCGCGGTCGACAACCAGGcgcg < 1:1435492/61‑1 (MQ=255) | GGCTATTTAACGCAAAAATTCTCCCGCGCGCTGGGTATGCTCGCGGTCGACAACCAGGCGCG > W3110S.gb/3551378‑3551439 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |