Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,578,721	T→C	T25T (ACT→ACC)	glnA →	glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,578,721	0	T	C	100.0%	249.1 / NA	73	T25T (ACT→ACC)	glnA	glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (73/0);  total (73/0)

CTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACTAAAGGTAAAGAAC  >  W3110S.gb/3578674‑3578734                                                |              cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCTCCGATACCAAAGGTAAAGAAc  >  1:1378278/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2650812/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2828768/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2810115/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2789590/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2734661/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2727630/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2713592/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2675328/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2662721/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2871830/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2558034/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2509704/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2504593/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2436146/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2429861/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2413508/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2258827/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2244952/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:687185/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:87026/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:843598/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:836991/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:794775/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:793077/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:759918/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:758102/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:743044/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2231222/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:64720/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:52313/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:489433/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:378160/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:366083/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:356607/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2879441/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1197605/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1020185/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1462328/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1427515/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1344783/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1319498/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1261414/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1249688/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1221763/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1465893/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1116362/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1096793/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1092233/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1083131/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1059323/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1049182/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1047475/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1031583/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1795709/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2162654/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2148276/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2007956/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1971871/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1966419/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1961300/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1950225/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:192537/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1854492/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:150045/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1790625/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1691546/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1644173/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1623925/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1614892/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1581744/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACAGATACCAAAGGTAAAGAAc  >  1:450645/1‑61 (MQ=255) cTGAACGAGCACGAAGTGAAGTTTGTTGATTAGCGCTTCACCGATACCAAAGGTAAAg     >  1:303259/1‑58 (MQ=255)                                                |              CTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACTAAAGGTAAAGAAC  >  W3110S.gb/3578674‑3578734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑