Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,582,332	G→A	P310P (CCG→CCA)	glnG →	fused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,582,332	0	G	A	100.0%	197.3 / NA	61	P310P (CCG→CCA)	glnG	fused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (61/0);  total (61/0)

CCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCATTTTTTACAGGTTGCC  >  W3110S.gb/3582281‑3582389                                                    |                                                          ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTg                                                        >  1:1288951/1‑55 (MQ=38) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGaa                                                  >  1:1153637/1‑61 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGaa                                                  >  1:961955/1‑61 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGaa                                                  >  1:288759/1‑61 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:2741564/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:462462/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:342371/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:319759/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:2899396/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:2846686/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:2817466/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:280332/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:2797734/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:490566/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:265395/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:2557661/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:2479838/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:2453987/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:2432794/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:488198/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:2451261/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:514010/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:653492/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:720621/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:748332/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:809319/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:815103/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:821219/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:858944/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:890607/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:903853/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:959694/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:975482/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:976036/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:1670763/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:1088665/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:1174711/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:1174896/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:1206203/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:1239763/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:1317682/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:1387103/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:1407405/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:1485425/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:1507065/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:1554520/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:1558966/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:1654479/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:1690019/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:1746101/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:2270161/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:2222200/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:2218958/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:1797701/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:1814558/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:1834188/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:2035052/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:2032180/1‑62 (MQ=255) ccGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTAATCTGCCGCCACTGCGCGAAc                                                 >  1:1794314/1‑62 (MQ=38) ccGTGAGGATCTGTTCCACAGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:2410989/1‑62 (MQ=38) ccGTGAGGATCAGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAAc                                                 >  1:663837/1‑62 (MQ=38)                                                 gccgcTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCATTTTTTTCAGGTTGcc  <  1:485358/61‑1 (MQ=255)                                                 gccgcTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCATTTTTTACAGGTTGcc  <  1:1454298/61‑1 (MQ=255)                                                 gccgcTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCATTTTTTACAGGTTGcc  <  1:1077047/61‑1 (MQ=255)                                                 gccgcTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCATTTTTTACAGGTTGcc  <  1:1252625/61‑1 (MQ=255)                                                 gccgcTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCATTTTTTACAGGTTGcc  <  1:209808/61‑1 (MQ=255)                                                 gccgcTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCATTTTTTACAGGTTGcc  <  1:1417139/61‑1 (MQ=255)                                                 gccgcTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCATTTTTTACAGGTTGcc  <  1:756585/61‑1 (MQ=255)                                                 gccgcTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCATTTTTTACAGGTTGcc  <  1:1784196/61‑1 (MQ=255)                                                 gccgcTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCATTTTTTACAGGTTGcc  <  1:1787687/61‑1 (MQ=255)                                                 gccgcTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCATTTTTTACAGGTTGcc  <  1:2355889/61‑1 (MQ=255)                                                 gccgcTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCATTTTTTACAGGTTGcc  <  1:374878/61‑1 (MQ=255)                                                 gccgcTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCATTTTTTACAGGTTGcc  <  1:358156/61‑1 (MQ=255)                                                 gccgcTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCATTTTTTACAGGTTGcc  <  1:288820/61‑1 (MQ=255)                                                    |                                                          CCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCATTTTTTACAGGTTGCC  >  W3110S.gb/3582281‑3582389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑