Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,584,262	G→A	R125R (CGC→CGT)	hemN ←	coproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,584,262	0	G	A	100.0%	220.4 / NA	65	R125R (CGC→CGT)	hemN	coproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/65);  total (0/65)

ATCGAAATCTCCGCATCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGGCGGCTGATTTGCGCTTTATT  >  W3110S.gb/3584206‑3584282                                                         |                     atcgaAATCTCCGCATCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAggcggc                  <  1:24272/61‑1 (MQ=255) atcgaAATCTCCGCATCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAggcggc                  <  1:1228595/61‑1 (MQ=255) atcgaAATCTCCGCATCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAggcggc                  <  1:2551349/61‑1 (MQ=255) atcgaAATCTCCGCATCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAggcggc                  <  1:1736632/61‑1 (MQ=255) atcgaAATCTCCGCATCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAggcggc                  <  1:2669170/61‑1 (MQ=255) atcgaAATCTCCGCATCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAggcggc                  <  1:657913/61‑1 (MQ=255)                tCGGGATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:2739443/62‑1 (MQ=255)                tCGGCTTTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:747923/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCCTCAGACGGCTGATTTGCGCTTTAtt  <  1:601987/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:350777/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:537417/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:326198/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:284483/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:2827088/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:2808251/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:2796335/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:2645655/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:2579815/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:2488318/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:2481500/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:2481008/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:471311/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:535795/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:2461630/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:538620/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:555645/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:63477/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:67304/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:683153/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:813148/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:844668/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:907701/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:919860/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:942090/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:958501/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:995632/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:2447623/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:1051/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:1061043/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:1130477/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:1210324/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:1259235/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:1380321/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:1393947/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:1430505/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:1525038/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:1630802/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:1645524/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:1687645/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:1707338/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:1749545/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:1879410/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:1023520/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:1893947/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:2365067/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:1925023/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:1933164/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:2045973/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:2071593/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:2093024/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:2279437/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:2153964/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:2192035/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:2208589/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:226412/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:2219697/62‑1 (MQ=255)                tCGGCATTGAACTGGAAGATTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:537000/62‑1 (MQ=255)                 cGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:884268/61‑1 (MQ=255)                 cGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:726952/61‑1 (MQ=255)                 cGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:1725085/61‑1 (MQ=255)                 cGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGACGGCTGATTTGCGCTTTAtt  <  1:18071/61‑1 (MQ=255)                                                         |                     ATCGAAATCTCCGCATCGGCATTGAACTGGAAGTTTTCGCGCAGCAGCTTCATCAGGCGGCTGATTTGCGCTTTATT  >  W3110S.gb/3584206‑3584282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑