| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,591,290 | A→G | V398A (GTC→GCC) | yihF ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,591,290 | 0 | A | G | 89.5% | 152.7 / 6.9 | 57 | V398A (GTC→GCC) | yihF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base G (0/51); total (6/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.76e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
TCCGCATCCATTCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGACGACATTAAGCGGTAACTTCACATCAAA > W3110S.gb/3591242‑3591318 | tCCGCATCCATCCCTTCATATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:2046320/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:33617/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:1099250/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:225504/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:2255147/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:2264264/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:234673/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:2527599/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:2543096/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:2614248/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:2726542/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:2745641/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:275287/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:2863446/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:2217127/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:363553/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:420163/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:586677/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:631926/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:802080/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:810696/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:85751/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:863787/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:896596/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:925167/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:950981/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:981868/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:998248/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:218754/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:1158370/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:1163785/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:1226942/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:1364080/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:1575011/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:1590872/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:1592763/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:1678055/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:1701953/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:1707083/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:1864088/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:1894295/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:2138464/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:2097444/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:2090032/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:2045647/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:1999865/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:1968530/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:1922506/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:1889560/60‑1 (MQ=255)tCCGCATCCATCCCTTCAGATAAATTAAGCTGTTCTGCGGTTTCTGTGGCGACATTAAGc < 1:751929/60‑1 (MQ=255) tCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGACGACATTAAGCGGTAACTTCACATCaaa > 1:147591/1‑62 (MQ=255) tCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGACGACATTAAGCGGTAACTTCACATCaaa > 1:1357367/1‑62 (MQ=255) tCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGACGACATTAAGCGGTAACTTCACATCaaa > 1:2113203/1‑62 (MQ=255) tCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGACGACATTAAGCGGTAACTTCACATCaaa > 1:1201666/1‑62 (MQ=255) tCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGACGACATTAAGCGGTAACTTCACATCaaa > 1:983011/1‑62 (MQ=255) tCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGACGACATTAAGCGGTAACTTCACATCaaa > 1:2202696/1‑62 (MQ=255) gATAAATTAAGCTGTTTTGCGGTTTCTGTGGCGACATTAAGc < 1:375075/42‑1 (MQ=255) | TCCGCATCCATTCCTTCAGATAAATTAAGCTGTTTTGCGGTTTCTGTGACGACATTAAGCGGTAACTTCACATCAAA > W3110S.gb/3591242‑3591318 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |