Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,615,349	A→G	P369P (CCT→CCC)	ubiB ←	2‑octaprenylphenol hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,615,349	0	A	G	100.0%	45.6 / NA	15	P369P (CCT→CCC)	ubiB	2‑octaprenylphenol hydroxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/15);  total (0/15)

TTTCTCAAAGATAGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGTAT  >  W3110S.gb/3615337‑3615398             |                                                  tttCTCAAAGATGGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGTAt  <  1:1048053/62‑1 (MQ=255) tttCTCAAAGATGGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGTAt  <  1:1076429/62‑1 (MQ=255) tttCTCAAAGATGGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGTAt  <  1:1097848/62‑1 (MQ=255) tttCTCAAAGATGGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGTAt  <  1:1655541/62‑1 (MQ=255) tttCTCAAAGATGGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGTAt  <  1:2284662/62‑1 (MQ=255) tttCTCAAAGATGGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGTAt  <  1:2335336/62‑1 (MQ=255) tttCTCAAAGATGGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGTAt  <  1:2556320/62‑1 (MQ=255) tttCTCAAAGATGGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGTAt  <  1:454494/62‑1 (MQ=255) tttCTCAAAGATGGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGTAt  <  1:525508/62‑1 (MQ=255) tttCTCAAAGATGGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGTAt  <  1:804299/62‑1 (MQ=255) gttCTCAAAGATGGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGTAt  <  1:2466609/61‑1 (MQ=255)  ttCTCAAAGATGGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGTAt  <  1:1526822/61‑1 (MQ=255)  ttCTCAAAGATGGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGTAt  <  1:1966245/61‑1 (MQ=255)  ttCTCAAAGATGGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGTAt  <  1:2041379/61‑1 (MQ=255)  ttCTCAAAGATGGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGTAt  <  1:299924/61‑1 (MQ=255)             |                                                  TTTCTCAAAGATAGGTTCACAGACCGTACGAATGGCAAATTCGAACTCTTCAACGTTGGTAT  >  W3110S.gb/3615337‑3615398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑