Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,675,341	T→A	S222C (AGC→TGC)	rep ←	DNA helicase and single‑stranded DNA‑dependent ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,675,341	0	T	A	100.0%	43.1 / NA	20	S222C (AGC→TGC)	rep	DNA helicase and single‑stranded DNA‑dependent ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (20/0);  total (20/0)

CCAGCAGTTTCACCAGCTCATACTGGCTGGTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAGCG  >  W3110S.gb/3675314‑3675398                            |                                                          ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt                                             >  1:2898602/1‑42 (MQ=19) ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt                                             >  1:2275134/1‑42 (MQ=19) ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt                                             >  1:2349785/1‑42 (MQ=19) ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt                                             >  1:2151663/1‑42 (MQ=19) ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt                                             >  1:2097116/1‑42 (MQ=19) ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt                                             >  1:2438582/1‑42 (MQ=19) ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt                                             >  1:1999855/1‑42 (MQ=19) ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt                                             >  1:2921049/1‑42 (MQ=19) ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt                                             >  1:1754213/1‑42 (MQ=19) ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt                                             >  1:1730205/1‑42 (MQ=19) ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt                                             >  1:1720946/1‑42 (MQ=19) ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt                                             >  1:1630491/1‑42 (MQ=19) ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt                                             >  1:1480185/1‑42 (MQ=19) ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt                                             >  1:2791/1‑42 (MQ=19) ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt                                             >  1:73529/1‑42 (MQ=19) ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt                                             >  1:1264389/1‑42 (MQ=19) ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt                                             >  1:745508/1‑42 (MQ=19) ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt                                             >  1:1227808/1‑42 (MQ=19) ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt                                             >  1:77495/1‑42 (MQ=19) ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt                                             >  1:900961/1‑42 (MQ=19)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATATCGAATTTTGTTCTGCCAgcg  <  1:402653/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:2734867/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:2849681/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:948802/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:326880/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:350166/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:352589/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:424683/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:494499/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:70896/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:1166724/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:1818466/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:1184240/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:1184950/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:1220967/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:1242758/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:1353351/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:1363022/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:1587326/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:1707098/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:2690957/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:1865536/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:2027867/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:220910/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:2487186/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:2510928/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:2582557/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:2626194/61‑1 (MQ=255)                         ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:2663636/61‑1 (MQ=255)                          gctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg  <  1:710750/60‑1 (MQ=255)                            |                                                          CCAGCAGTTTCACCAGCTCATACTGGCTGGTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAGCG  >  W3110S.gb/3675314‑3675398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑