Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,683,940 C→T V86V (GTG→GTA)  ilvE ← branched‑chain amino‑acid aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,683,9400CT100.0% 152.8 / NA 47V86V (GTG→GTA) ilvEbranched‑chain amino‑acid aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/47);  total (0/47)

CGACGAAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATCACGTCACGACAAGCTTCCATCAGCTCATCAATGCT  >  W3110S.gb/3683888‑3683975
                                                    |                                   
cgacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg                             <  1:167209/61‑1 (MQ=255)
cgacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg                             <  1:45854/61‑1 (MQ=255)
cgacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg                             <  1:507467/61‑1 (MQ=255)
cgacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg                             <  1:1910585/61‑1 (MQ=255)
cgacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg                             <  1:706394/61‑1 (MQ=255)
cgacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg                             <  1:1907584/61‑1 (MQ=255)
cgacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg                             <  1:355462/61‑1 (MQ=255)
cgacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg                             <  1:204247/61‑1 (MQ=255)
cgacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg                             <  1:2154468/61‑1 (MQ=255)
cgacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg                             <  1:2280386/61‑1 (MQ=255)
cgacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg                             <  1:2768175/61‑1 (MQ=255)
cgacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg                             <  1:106803/61‑1 (MQ=255)
 gacgaAGATCAGCGGACGGATATAGGCGCTGGTTAGATTGTTTTTGCGGAttacgtcacg                             <  1:1191614/60‑1 (MQ=255)
 gacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg                             <  1:1220794/60‑1 (MQ=255)
  acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa                          <  1:2718925/62‑1 (MQ=255)
  acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa                          <  1:2107328/62‑1 (MQ=255)
  acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa                          <  1:29173/62‑1 (MQ=255)
  acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa                          <  1:2359483/62‑1 (MQ=255)
  acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa                          <  1:434274/62‑1 (MQ=255)
  acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa                          <  1:2764367/62‑1 (MQ=255)
  acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa                          <  1:2678611/62‑1 (MQ=255)
  acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa                          <  1:2706190/62‑1 (MQ=255)
  acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa                          <  1:1877114/62‑1 (MQ=255)
  acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa                          <  1:1824109/62‑1 (MQ=255)
  acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa                          <  1:1756743/62‑1 (MQ=255)
  acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa                          <  1:1702714/62‑1 (MQ=255)
  acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa                          <  1:1685051/62‑1 (MQ=255)
  acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa                          <  1:1363037/62‑1 (MQ=255)
  acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa                          <  1:710186/62‑1 (MQ=255)
  acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa                          <  1:754419/62‑1 (MQ=255)
  acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa                          <  1:774999/62‑1 (MQ=255)
  acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa                          <  1:1117628/62‑1 (MQ=255)
  acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa                          <  1:1005000/62‑1 (MQ=255)
          cAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg                             <  1:2843831/51‑1 (MQ=255)
             cggacggaTATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCa               <  1:2196072/62‑1 (MQ=255)
                 cggaTATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCtc           <  1:932494/62‑1 (MQ=255)
                 cggaTATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCtc           <  1:1045016/62‑1 (MQ=255)
                 cggaTATACGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCtc           <  1:337436/62‑1 (MQ=255)
                           cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct  <  1:1943443/61‑1 (MQ=255)
                           cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct  <  1:94103/61‑1 (MQ=255)
                           cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct  <  1:1083396/61‑1 (MQ=255)
                           cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct  <  1:928589/61‑1 (MQ=255)
                           cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct  <  1:918543/61‑1 (MQ=255)
                           cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct  <  1:1199653/61‑1 (MQ=255)
                           cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct  <  1:1257558/61‑1 (MQ=255)
                           cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct  <  1:1908486/61‑1 (MQ=255)
                           cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct  <  1:1930577/61‑1 (MQ=255)
                           cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct  <  1:48082/61‑1 (MQ=255)
                           cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct  <  1:48013/61‑1 (MQ=255)
                           cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct  <  1:2638656/61‑1 (MQ=255)
                           cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct  <  1:1970445/61‑1 (MQ=255)
                           cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct  <  1:363186/61‑1 (MQ=255)
                           cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct  <  1:33515/61‑1 (MQ=255)
                           cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct  <  1:329500/61‑1 (MQ=255)
                           cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct  <  1:2065486/61‑1 (MQ=255)
                           cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct  <  1:2881777/61‑1 (MQ=255)
                           cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct  <  1:2166465/61‑1 (MQ=255)
                           cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct  <  1:2791127/61‑1 (MQ=255)
                           cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct  <  1:2540142/61‑1 (MQ=255)
                            gcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct  <  1:1160564/60‑1 (MQ=255)
                                 tGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct  <  1:620999/55‑1 (MQ=255)
                                     aTTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct  <  1:1985203/51‑1 (MQ=255)
                                                    |                                   
CGACGAAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATCACGTCACGACAAGCTTCCATCAGCTCATCAATGCT  >  W3110S.gb/3683888‑3683975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑