| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 32,559 | T→C | C581C (TGT→TGC) | carB → | carbamoyl‑phosphate synthase large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 32,559 | 0 | T | C | 96.6% | 284.2 / ‑0.8 | 89 | C581C (TGT→TGC) | carB | carbamoyl‑phosphate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base T (3/0); new base C (86/0); total (89/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
GTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTA > W3110S.gb/32540‑32608 | gTATCGAATTCGAGTACTGCTGTGTACACGCCTCGCTgg > 1:1553177/1‑39 (MQ=37)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:401247/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:408782/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:358268/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:338380/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:333166/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2924838/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2887503/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2857151/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2825085/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:280680/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2795909/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2693028/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2691101/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2661046/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2652294/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2644282/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2608407/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2554804/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2549752/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2536064/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2449015/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2445949/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:813182/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:983049/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:965133/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:935797/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:930910/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:924066/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:921887/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:918730/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:822328/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:820047/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:815016/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2424684/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:809403/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:758040/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:716412/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:710124/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:670113/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:641287/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:614589/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:517199/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:444518/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:1580392/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:1813078/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:1744183/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:1742259/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:1701740/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:1697791/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:1683685/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:168191/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:1657363/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:1644179/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:16105/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2295385/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:1548361/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:1431681/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:1400457/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:1320303/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:1256763/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:1227765/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:1184346/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:1177451/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:1114195/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2014967/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2191267/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:218580/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2185630/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2175931/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2135388/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2070253/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2193380/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2048841/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:2264131/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:1971380/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:1916238/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:1017880/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:1830699/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:1904745/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:1885254/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTgg > 1:1853485/1‑39 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTg > 1:2169246/1‑38 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTCGCTg > 1:607066/1‑38 (MQ=38)gTATCGAATTCGACTACTGCTGTGTACACGCCTAGCTgg > 1:1078535/1‑39 (MQ=37)gTATCGAATTCGACTACTGCTGTGTACACGCCACGCTgg > 1:1915844/1‑39 (MQ=37) aTTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTa > 1:2065911/1‑62 (MQ=255) aTTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTa > 1:1675800/1‑62 (MQ=255) aTTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTa > 1:626536/1‑62 (MQ=255) | GTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTA > W3110S.gb/32540‑32608 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |