Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,722,563	G→A	A361T (GCT→ACT)	glmU →	fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase and glucosamine‑1‑phosphate acetyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,722,563	0	G	A	100.0%	44.3 / NA	15	A361T (GCT→ACT)	glmU	fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase and glucosamine‑1‑phosphate acetyl transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/8);  total (7/8)

CACGTCGGTAACTTCGTTGAGATGAAAAAAGCGCGTCTGGGTAAAGGCTCGAAAGCTGGTCAT  >  W3110S.gb/3722509‑3722571                                                       |         cACGTCGGTAACTTCGTTGAGATGAAAAAAGCGCGTCTGGGTAAAGGCTCGAAAACTGGTCa   >  1:1296945/1‑62 (MQ=255) cACGTCGGTAACTTCGTTGAGATGAAAAAAGCGCGTCTGGGTAAAGGCTCGAAAACTGGTCa   >  1:1597109/1‑62 (MQ=255) cACGTCGGTAACTTCGTTGAGATGAAAAAAGCGCGTCTGGGTAAAGGCTCGAAAACTGGTCa   >  1:2014054/1‑62 (MQ=255) cACGTCGGTAACTTCGTTGAGATGAAAAAAGCGCGTCTGGGTAAAGGCTCGAAAACTGGTCa   >  1:2186250/1‑62 (MQ=255) cACGTCGGTAACTTCGTTGAGATGAAAAAAGCGCGTCTGGGTAAAGGCTCGAAAACTGGTCa   >  1:2649117/1‑62 (MQ=255) cACGTCGGTAACTTCGTTGAGATGAAAAAAGCGCGTCTGGGTAAAGGCTCGAAAACTGGTCa   >  1:2699095/1‑62 (MQ=255) cACGTCGGTAACTTCGTTGAGATGAAAAAAGCGCGTCTGGGTAAAGGCTCGAAAACTGGTCa   >  1:477831/1‑62 (MQ=255)    gTCGGTAACTTCGTTGAGATGAAAAAAGCGCGTCTGGGTAAAGGCTCGAAAACTGGTCAt  <  1:1484080/60‑1 (MQ=255)    gTCGGTAACTTCGTTGAGATGAAAAAAGCGCGTCTGGGTAAAGGCTCGAAAACTGGTCAt  <  1:1971631/60‑1 (MQ=255)    gTCGGTAACTTCGTTGAGATGAAAAAAGCGCGTCTGGGTAAAGGCTCGAAAACTGGTCAt  <  1:2878099/60‑1 (MQ=255)    gTCGGTAACTTCGTTGAGATGAAAAAAGCGCGTCTGGGTAAAGGCTCGAAAACTGGTCAt  <  1:2879413/60‑1 (MQ=255)    gTCGGTAACTTCGTTGAGATGAAAAAAGCGCGTCTGGGTAAAGGCTCGAAAACTGGTCAt  <  1:414008/60‑1 (MQ=255)    gTCGGTAACTTCGTTGAGATGAAAAAAGCGCGTCTGGGTAAAGGCTCGAAAACTGGTCAt  <  1:522745/60‑1 (MQ=255)    gTCGGTAACTTCGTTGAGATGAAAAAAGCGCGTCTGGGTAAAGGCTCGAAAACTGGTCAt  <  1:585174/60‑1 (MQ=255)    gTCGGTAACTTCGTTGAGATGAAAAAAGCGCGTCTGGGTAAAGGCTCGAAAACTGGTCAt  <  1:695225/60‑1 (MQ=255)                                                       |         CACGTCGGTAACTTCGTTGAGATGAAAAAAGCGCGTCTGGGTAAAGGCTCGAAAGCTGGTCAT  >  W3110S.gb/3722509‑3722571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑