| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,726,860 | G→A | V192V (GTG→GTA) | pstC → | phosphate transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,726,860 | 0 | G | A | 75.4% | 100.4 / 33.6 | 61 | V192V (GTG→GTA) | pstC | phosphate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/15); new base A (46/0); total (46/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.42e-14 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
TCGCCATCATGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTG > W3110S.gb/3726814‑3726919 | tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAAcccc > 1:467617/1‑61 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2792104/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1833312/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:354675/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1924410/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:332819/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:314329/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2867021/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2854933/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2132095/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2193854/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2216131/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1829973/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2787044/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2236491/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2602307/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2268969/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2554861/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2398544/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2403897/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:254491/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2480572/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2539267/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1488706/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:903372/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:887152/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:840822/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1197247/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:83259/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1332728/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1339519/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1365881/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1397824/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1452019/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:14735/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:508960/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1571038/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1584321/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:481196/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1710830/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:464419/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1767730/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1776457/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:38030/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAAAAAACCCCg > 1:1914358/1‑62 (MQ=255)tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATACGAAAAAAccc > 1:1024131/1‑60 (MQ=255) tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTcag < 1:1585438/61‑3 (MQ=255) tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:1961214/61‑1 (MQ=255) tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:57615/61‑1 (MQ=255) tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:1491982/61‑1 (MQ=255) tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:165388/61‑1 (MQ=255) tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:253329/61‑1 (MQ=255) tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:409664/61‑1 (MQ=255) tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:609550/61‑1 (MQ=255) tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:1795220/61‑1 (MQ=255) tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:124949/61‑1 (MQ=255) tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:2233612/61‑1 (MQ=255) tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:2259217/61‑1 (MQ=255) tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:100148/61‑1 (MQ=255) tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:977952/61‑1 (MQ=255) tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgc < 1:104882/61‑2 (MQ=255) tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCgg > 1:59882/1‑36 (MQ=255) tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCCCCACCt > 1:307217/1‑60 (MQ=255) tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCt > 1:1975505/1‑60 (MQ=255) tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:422435/1‑61 (MQ=255) tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:751394/1‑61 (MQ=255) tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:758604/1‑61 (MQ=255) tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:1046567/1‑61 (MQ=255) tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:579042/1‑61 (MQ=255) tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:1749415/1‑61 (MQ=255) tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:402154/1‑61 (MQ=255) tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:1886022/1‑61 (MQ=255) tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:303767/1‑61 (MQ=255) tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:2092590/1‑61 (MQ=255) tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:2229424/1‑61 (MQ=255) tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:2325238/1‑61 (MQ=255) tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:2450041/1‑61 (MQ=255) tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACATg > 1:211780/1‑61 (MQ=255) | TCGCCATCATGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTG > W3110S.gb/3726814‑3726919 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |