Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,744,316	G→A	N313N (AAC→AAT)	yidZ ←	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,744,316	0	G	A	87.5%	54.9 / 1.3	24	N313N (AAC→AAT)	yidZ	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base A (21/0);  total (21/3)
Fisher's exact test for biased strand distribution p-value = 4.94e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

TCGCGTTTCATACGATTCGGTTATGCCATCGACGCGTAAAGGTTTTTAATGGTTTCCCGTAACCAGACGA  >  W3110S.gb/3744275‑3744344                                          |                             tCGCGTTTCATACGATTCGGTTATGCCATCGACGCGTAAAGGTTTTTAATGGTTTCCCGTaa          <  1:1659852/62‑1 (MQ=255) tCGCGTTTCATACGATTCGGTTATGCCATCGACGCGTAAAGGTTTTTAATGGTTTCCCGTaa          <  1:1838378/62‑1 (MQ=255) tCGCGTTTCATACGATTCGGTTATGCCATCGACGCGTAAAGGTTTTTAATGGTTTCCCGTaa          <  1:453758/62‑1 (MQ=255)         cATACGATTCGGTTTTGCCATCGACGCGTAAAGATTTTTAATGGTTTCCCGTAACCAGACGa  >  1:2055675/1‑62 (MQ=255)         cATACGATTCGGTTATGCCATCGACGCGTAAAGATTTTTAATGGttt                 >  1:1444354/1‑47 (MQ=255)         cATACGATTCGGTTATGCCATCGACGCGTAAAGATTTTTAATGGtt                  >  1:2125821/1‑46 (MQ=255)         cATACGATTCGGTTATGCCATCGACGCGTAAAGATTTTTAATGGTTTCCCGTAACCAGACg   >  1:2794628/1‑61 (MQ=255)         cATACGATTCGGTTATGCCATCGACGCGTAAAGATTTTTAATGGTTTCCCGTAACCAGACGa  >  1:950846/1‑62 (MQ=255)         cATACGATTCGGTTATGCCATCGACGCGTAAAGATTTTTAATGGTTTCCCGTAACCAGACGa  >  1:1222555/1‑62 (MQ=255)         cATACGATTCGGTTATGCCATCGACGCGTAAAGATTTTTAATGGTTTCCCGTAACCAGACGa  >  1:853890/1‑62 (MQ=255)         cATACGATTCGGTTATGCCATCGACGCGTAAAGATTTTTAATGGTTTCCCGTAACCAGACGa  >  1:848116/1‑62 (MQ=255)         cATACGATTCGGTTATGCCATCGACGCGTAAAGATTTTTAATGGTTTCCCGTAACCAGACGa  >  1:718730/1‑62 (MQ=255)         cATACGATTCGGTTATGCCATCGACGCGTAAAGATTTTTAATGGTTTCCCGTAACCAGACGa  >  1:51770/1‑62 (MQ=255)         cATACGATTCGGTTATGCCATCGACGCGTAAAGATTTTTAATGGTTTCCCGTAACCAGACGa  >  1:2502082/1‑62 (MQ=255)         cATACGATTCGGTTATGCCATCGACGCGTAAAGATTTTTAATGGTTTCCCGTAACCAGACGa  >  1:2299834/1‑62 (MQ=255)         cATACGATTCGGTTATGCCATCGACGCGTAAAGATTTTTAATGGTTTCCCGTAACCAGACGa  >  1:2248993/1‑62 (MQ=255)         cATACGATTCGGTTATGCCATCGACGCGTAAAGATTTTTAATGGTTTCCCGTAACCAGACGa  >  1:211986/1‑62 (MQ=255)         cATACGATTCGGTTATGCCATCGACGCGTAAAGATTTTTAATGGTTTCCCGTAACCAGACGa  >  1:2043253/1‑62 (MQ=255)         cATACGATTCGGTTATGCCATCGACGCGTAAAGATTTTTAATGGTTTCCCGTAACCAGACGa  >  1:2006165/1‑62 (MQ=255)         cATACGATTCGGTTATGCCATCGACGCGTAAAGATTTTTAATGGTTTCCCGTAACCAGACGa  >  1:1927428/1‑62 (MQ=255)         cATACGATTCGGTTATGCCATCGACGCGTAAAGATTTTTAATGGTTTCCCGTAACCAGACGa  >  1:1923581/1‑62 (MQ=255)         cATACGATTCGGTTATGCCATCGACGCGTAAAGATTTTTAATGGTTTCCCGTAACCAGACGa  >  1:1806471/1‑62 (MQ=255)         cATACGATTCGGTTATGCCATCGACGCGTAAAGATTTTTAATGGTTTCCCGTAACCAGACGa  >  1:1374205/1‑62 (MQ=255)         cATACGATTCGGTTATGCCATCGACGCGTAAAGAATTTTAATGGTTTCCCGTAACCAGAcca  >  1:539505/1‑60 (MQ=255)                                          |                             TCGCGTTTCATACGATTCGGTTATGCCATCGACGCGTAAAGGTTTTTAATGGTTTCCCGTAACCAGACGA  >  W3110S.gb/3744275‑3744344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑