Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,770,855	C→T	G62G (GGG→GGA)	cbrA ←	predicted oxidoreductase, FAD/NAD(P)‑binding domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,770,855	0	C	T	100.0%	161.4 / NA	50	G62G (GGG→GGA)	cbrA	predicted oxidoreductase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/50);  total (0/50)

GTCGACGGTTTTGACGCTGAAAATCTGCGGATTAGCGATCACATCGACAGGAAGCGTCAGCCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCCGCAGGGTTTGC  >  W3110S.gb/3770795‑3770913                                                             |                                                           gTCGACGGTTTTGACGCTGAAAATCTGCGGATTAGCGATCACATCGACAGGAAGCGTCAGcc                                                           <  1:1053244/62‑1 (MQ=255) gTCGACGGTTTTGACGCTGAAAATCTGCGGATTAGCGATCACATCGACAGGAAGCGTCAGcc                                                           <  1:2457247/62‑1 (MQ=255) gTCGACGGTTTTGACGCTGAAAATCTGCGGATTAGCGATCACATCGACAGGAAGCGTCAGcc                                                           <  1:2791736/62‑1 (MQ=255) gTCGACGGTTTTGACGCTGAAAATCTGCGGATTAGCGATCACATCGACAGGAAGCGTCAGcc                                                           <  1:2810297/62‑1 (MQ=255) gTCGACGGTTTTGACGCTGAAAATCTGCGGATTAGCGATCACATCGACAGGAAGCGTCAGcc                                                           <  1:2816093/62‑1 (MQ=255) gTCGACGGTTTTGACGCTGAAAATCTGCGGATTAGCGATCACATCGACAGGAAGCGTCAGcc                                                           <  1:2068309/62‑1 (MQ=255) gTCGACGGTTTTGACGCTGAAAATCTGCGGATTAGCGATCACATCGACAGGAAGCGTCAGcc                                                           <  1:364479/62‑1 (MQ=255) gTCGACGGTTTTGACGCTGAAAATCTGCGGATTAGCGATCACATCGACAGGAAGCGTCAGcc                                                           <  1:194829/62‑1 (MQ=255) gTCGACGGTTTTGACGCTGAAAATCTGCGGATTAGCGATCACATCGACAGGAAGCGTCAGcc                                                           <  1:1911966/62‑1 (MQ=255) gTCGACGGTTTTGACGCTGAAAATCTGCGGATTAGCGATCACATCGACAGGAAGCGTCAGcc                                                           <  1:1868441/62‑1 (MQ=255) gTCGACGGTTTTGACGCTGAAAATCTGCGGATTAGCGATCACATCGACAGGAAGCGTCAGcc                                                           <  1:401264/62‑1 (MQ=255) gTCGACGGTTTTGACGCTGAAAATCTGCGGATTAGCGATCACATCGACAGGAAGCGTCAGcc                                                           <  1:514981/62‑1 (MQ=255) gTCGACGGTTTTGACGCTGAAAATCTGCGGATTAGCGATCACATCGACAGGAAGCGTCAGcc                                                           <  1:176412/62‑1 (MQ=255) gTCGACGGTTTTGACGCTGAAAATCTGCGGATTAGCGATCACATCGACAGGAAGCGTCAGcc                                                           <  1:541104/62‑1 (MQ=255) gTCGACGGTTTTGACGCTGAAAATCTGCGGATTAGCGATCACATCGACAGGAAGCGTCAGcc                                                           <  1:1503829/62‑1 (MQ=255) gTCGACGGTTTTGACGCTGAAAATCTGCGGATTAGCGATCACATCGACAGGAAGCGTCAGcc                                                           <  1:848520/62‑1 (MQ=255) gTCGACGGTTTTGACGCTGAAAATCTGCGGATTAGCGATCACATCGACAGGAAGCGTCAGcc                                                           <  1:629622/62‑1 (MQ=255) gTCGACGGTTTTGACGCTGAAAATCTGCGGATTAGCGATCACATCGACAGGAAGCGTCAGcc                                                           <  1:1304439/62‑1 (MQ=255) gTCGACGGTTTTGACGCTGAAAATCTGCGGATTAGCGATCACATCGACAGGAAGCGTCAGcc                                                           <  1:768110/62‑1 (MQ=255) gTCGACGGTTTTGACGCTGAAAATCTGCGGATTAGCGATCACATCGACAGGAAGCGTCAGcc                                                           <  1:1212365/62‑1 (MQ=255)  tCGACGGTTTTGACGCTGAAAATCTGCGGATTAGCGATCACATCGACAGGAAGCGTCAGcc                                                           <  1:763067/61‑1 (MQ=255)       ggTTTTGACGCTGAAAATCTGCGGATTAGCGATCACATCGACAGGAAGCGTCAGcc                                                           <  1:2674377/56‑1 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:604651/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:595967/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:2697043/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:864473/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:2697977/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:2705590/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:2706001/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:2763013/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:2763108/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:1001091/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:386055/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:645852/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:2824948/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:2841289/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:2914888/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:2948/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:397532/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:309442/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:647247/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:667343/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:1944957/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:1059996/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:1185540/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:11856/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:1186056/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:1231572/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:124030/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:1256034/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:1349161/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:1424094/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:1759942/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:1794815/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:1861894/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:1886165/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:2514438/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:2202346/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:2470350/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:2453413/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:2364694/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:2362445/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:2259457/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:2243383/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:197734/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:2136620/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:2103217/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:2085035/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:2072575/62‑3 (MQ=255)                                                    aaGCGTCAGTCCATCGCGAATAAAAGATCCCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:2283192/62‑3 (MQ=255)                                                     aGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:2374849/61‑3 (MQ=255)                                                     aGCGTCAGTCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCcacaag        <  1:376720/61‑3 (MQ=255)                                                             cccATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCCGCAGGGTTTGc  <  1:5998/59‑1 (MQ=255)                                                             cccATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCCGCAGGGTTTGc  <  1:1564319/59‑1 (MQ=255)                                                             cccATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCCGCAGGGTTTGc  <  1:661710/59‑1 (MQ=255)                                                             cccATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCCGCAGGGTTTGc  <  1:2024908/59‑1 (MQ=255)                                                             |                                                           GTCGACGGTTTTGACGCTGAAAATCTGCGGATTAGCGATCACATCGACAGGAAGCGTCAGCCCATCGCGAATAAAAGATCGCTGCGCGTCCGGTGCCAGCAGACCGCCGCAGGGTTTGC  >  W3110S.gb/3770795‑3770913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑