Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,785,542	C→T	P318P (CCG→CCA)	emrD ←	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,785,542	0	C	T	100.0%	177.7 / NA	54	P318P (CCG→CCA)	emrD	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/54);  total (0/54)

CAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTCGCCAGCGGAAACAGCATCCCGGCACCGA  >  W3110S.gb/3785503‑3785564                                        |                       cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:2534508/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1884061/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1020296/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1945833/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:2254184/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:2267443/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:2271857/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:2284341/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:230355/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:2351913/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:2371144/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:2374356/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:2433088/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1900237/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:2576555/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:2593597/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:2755685/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:2783014/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:2913259/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:315108/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:401178/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:671763/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:714461/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:789413/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:839585/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1698914/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1397267/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1500898/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1521643/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1366765/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1574463/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1589962/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1597787/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1270428/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1649077/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1213817/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1698958/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1700134/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1707003/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:170821/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1714339/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1773607/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1791120/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1867358/62‑1 (MQ=255) cAGGAAGGGGAACGGCTCCATCGCGCCGCTGGAAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1822164/62‑1 (MQ=255)  aGGAAGGGGAACGGCTCCATCGCGCCTCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:653416/61‑1 (MQ=255)  aGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:2591139/61‑1 (MQ=255)  aGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1645544/61‑1 (MQ=255)  aGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:2346120/61‑1 (MQ=255)  aGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1162271/61‑1 (MQ=255)  aGGAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1079027/61‑1 (MQ=255)    gAAGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1384718/59‑1 (MQ=255)      aGGGGAACGGCTCCATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:280751/57‑1 (MQ=255)                  ccATCGCGCCGCTGGTAGCCAGTGGAAACAGCATCCCGGCACCGa  <  1:1462790/45‑1 (MQ=38)                                        |                       CAGGAAGGGGAACGGCTCCATCGCGCCGCTGGTCGCCAGCGGAAACAGCATCCCGGCACCGA  >  W3110S.gb/3785503‑3785564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑