Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,836,867	A→G	K206K (AAA→AAG)	rfaB →	UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,836,867	0	A	G	100.0%	32.0 / NA	11	K206K (AAA→AAG)	rfaB	UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/11);  total (0/11)

GAAACGGCAACGTTTATTTATGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATCACAAGC  >  W3110S.gb/3836808‑3836893                                                            |                           gAAACGGCAACGTTTATTTATGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAGGa                          <  1:1539123/62‑1 (MQ=255) gAAACGGCAACGTTTATTTATGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAGGa                          <  1:1582463/62‑1 (MQ=255) gAAACGGCAACGTTTATTTATGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAGGa                          <  1:2013546/62‑1 (MQ=255) gAAACGGCAACGTTTATTTATGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAGGa                          <  1:369287/62‑1 (MQ=255) gAAACGGCAACGTTTATTTATGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAGGa                          <  1:408942/62‑1 (MQ=255) gAAACGGCAACGTTTATTTATGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAGGa                          <  1:743750/62‑1 (MQ=255)  aaaCGGCAACGTTTATTTATGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAGGa                          <  1:2434129/61‑1 (MQ=255)    aCGGCAACGTTTATTTATGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAGGa                          <  1:507026/59‑1 (MQ=255)                         gggCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAGGACCTGCTTGATGGCTTATCACAAGc  <  1:2304535/62‑1 (MQ=255)                         gggCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAGGACCTGCTTGATGGCTTATCACAAGc  <  1:2432364/62‑1 (MQ=255)                         gggCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAGGACCTGCTTGATGGCTTATCACAAGc  <  1:2453285/62‑1 (MQ=255)                                                            |                           GAAACGGCAACGTTTATTTATGTTGGGCGTATGAAATTTGAAGGCCAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATCACAAGC  >  W3110S.gb/3836808‑3836893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑