Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,900,169	T→C	M182V (ATG→GTG)	avtA ←	valine‑pyruvate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,900,169	0	T	C	100.0%	117.5 / NA	35	M182V (ATG→GTG)	avtA	valine‑pyruvate aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/13);  total (22/13)

TAATCACATTGCCTGTTGGATTCGTCGGCCGGGAGACGCAAATCATCCCGGTTTCTTCGCCAATATGCAGATGCTCAAAATCGACG  >  W3110S.gb/3900124‑3900209                                              |                                         tAATCACATTGCCTGTTGGATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCa                          <  1:1050804/62‑1 (MQ=255) tAATCACATTGCCTGTTGGATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCa                          <  1:771126/62‑1 (MQ=255) tAATCACATTGCCTGTTGGATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCa                          <  1:655747/62‑1 (MQ=255) tAATCACATTGCCTGTTGGATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCa                          <  1:638288/62‑1 (MQ=255) tAATCACATTGCCTGTTGGATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCa                          <  1:487339/62‑1 (MQ=255) tAATCACATTGCCTGTTGGATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCa                          <  1:2854334/62‑1 (MQ=255) tAATCACATTGCCTGTTGGATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCa                          <  1:273397/62‑1 (MQ=255) tAATCACATTGCCTGTTGGATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCa                          <  1:2495625/62‑1 (MQ=255) tAATCACATTGCCTGTTGGATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCa                          <  1:2402965/62‑1 (MQ=255) tAATCACATTGCCTGTTGGATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCa                          <  1:2001985/62‑1 (MQ=255) tAATCACATTGCCTGTTGGATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCa                          <  1:1926003/62‑1 (MQ=255) tAATCACATTGCCTGTTGGATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCa                          <  1:152141/62‑1 (MQ=255) tAATCACATTGCCTGTTGGATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCa                          <  1:161892/62‑1 (MQ=255)            ccTGTTGGATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCAATATGCAGATg               >  1:1977344/1‑62 (MQ=255)            ccTGTTGGATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCAATATGCAGATg               >  1:1423049/1‑62 (MQ=255)            ccTGTTGGATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCAATATGCAGATg               >  1:1462241/1‑62 (MQ=255)            ccTGTTGGATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCAATATGCAGATg               >  1:2603757/1‑62 (MQ=255)            ccTGTTGGATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCAATATGCAGATg               >  1:2225928/1‑62 (MQ=255)            ccTGTTGGATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCAATATGCAGATg               >  1:1793095/1‑62 (MQ=255)                  ggATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCAATATGCAGATGCTCaaa         >  1:473009/1‑62 (MQ=255)                  ggATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCAATATGCAGATGCTCaaa         >  1:901815/1‑62 (MQ=255)                  ggATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCAATATGCAGATGCTCaaa         >  1:898857/1‑62 (MQ=255)                  ggATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCAATATGCAGATGCTCaaa         >  1:772993/1‑62 (MQ=255)                  ggATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCAATATGCAGATGCTCaaa         >  1:1109895/1‑62 (MQ=255)                  ggATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCAATATGCAGATGCTCaaa         >  1:1435198/1‑62 (MQ=255)                  ggATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCAATATGCAGATGCTCaaa         >  1:1692259/1‑62 (MQ=255)                  ggATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCAATATGCAGATGCTCaaa         >  1:2437008/1‑62 (MQ=255)                  ggATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCAATATGCAGATGCTCaa          >  1:704049/1‑61 (MQ=255)                  ggATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCAATATGCAGATGCTCaa          >  1:1008589/1‑61 (MQ=255)                  ggATTCGTCGGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCAATATGCAGATGCTCa           >  1:2620620/1‑60 (MQ=255)                  ggATTCGTCGGCCGGGAGACACAAATCACCCCGGTTTCTTCGCCAATATGCAGATGCTCaaa         >  1:465631/1‑62 (MQ=255)                         tcgGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCAATATGCAGATGCTCAAAATCGACg  >  1:356042/1‑62 (MQ=255)                         tcgGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCAATATGCAGATGCTCAAAATCGACg  >  1:2087440/1‑62 (MQ=255)                         tcgGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCAATATGCAGATGCTCAAAATCGACg  >  1:2275567/1‑62 (MQ=255)                         tcgGCCGGGAGACGCAAATCACCCCGGTTTCTTCGCCAATATGCAGATGCTCAAAATCGACg  >  1:2383821/1‑62 (MQ=255)                                              |                                         TAATCACATTGCCTGTTGGATTCGTCGGCCGGGAGACGCAAATCATCCCGGTTTCTTCGCCAATATGCAGATGCTCAAAATCGACG  >  W3110S.gb/3900124‑3900209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑