Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,911,270 C→A G75G (GGC→GGA)  xylB → xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,911,2700CA77.5% 155.4 / 80.2 120G75G (GGC→GGA) xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base C (0/27);  new base A (93/0);  total (93/27)
Fisher's exact test for biased strand distribution p-value = 1.88e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

Only 100 of 120 total aligned reads displayed.

AGCATTCTCTGCAGGACGTTAAAGCATTGGGTATTGCCGGCCAGATGCACGGAGCAACCTTG  >  W3110S.gb/3911230‑3911291
                                        |                     
aGCATTCTCTGCAGGACGTTAAAGCATTGGGTATTGCCGGCCAGATGCACTGAGCAACCTTg  <  1:2556314/62‑1 (MQ=255)
aGCATTCTCTGCAGGACGTTAAAGCATTGGGTATTGCCGGCCAGATGCACGGAGCAACCTTg  <  1:1662772/62‑1 (MQ=255)
aGCATTCTCTGCAGGACGTTAAAGCATTGGGTATTGCCGGCCAGATGCACGGAGCAACCTTg  <  1:2357806/62‑1 (MQ=255)
aGCATTCTCTGCAGGACGTTAAAGCATTGGGTATTGCCGGCCAGATGCACGGAGCAACCTTg  <  1:2301885/62‑1 (MQ=255)
aGCATTCTCTGCAGGACGTTAAAGCATTGGGTATTGCCGGCCAGATGCACGGAGCAACCTTg  <  1:2270199/62‑1 (MQ=255)
aGCATTCTCTGCAGGACGTTAAAGCATTGGGTATTGCCGGCCAGATGCACGGAGCAACCTTg  <  1:1946812/62‑1 (MQ=255)
aGCATTCTCTGCAGGACGTTAAAGCATTGGGTATTGCCGGCCAGATGCACGGAGCAACCTTg  <  1:1898790/62‑1 (MQ=255)
aGCATTCTCTGCAGGACGTTAAAGCATTGGGTATTGCCGGCCAGATGCACGGAGCAACCTTg  <  1:1876486/62‑1 (MQ=255)
aGCATTCTCTGCAGGACGTTAAAGCATTGGGTATTGCCGGCCAGATGCACGGAGCAACCTTg  <  1:1873025/62‑1 (MQ=255)
aGCATTCTCTGCAGGACGTTAAAGCATTGGGTATTGCCGGCCAGATGCACGGAGCAACCTTg  <  1:1829861/62‑1 (MQ=255)
aGCATTCTCTGCAGGACGTTAAAGCATTGGGTATTGCCGGCCAGATGCACGGAGCAACCTTg  <  1:2856528/62‑1 (MQ=255)
aGCATTCTCTGCAGGACGTTAAAGCATTGGGTATTGCCGGCCAGATGCACGGAGCAACCTTg  <  1:2918328/62‑1 (MQ=255)
aGCATTCTCTGCAGGACGTTAAAGCATTGGGTATTGCCGGCCAGATGCACGGAGCAACCTTg  <  1:377566/62‑1 (MQ=255)
aGCATTCTCTGCAGGACGTTAAAGCATTGGGTATTGCCGGCCAGATGCACGGAGCAACCTTg  <  1:1481360/62‑1 (MQ=255)
aGCATTCTCTGCAGGACGTTAAAGCATTGGGTATTGCCGGCCAGATGCACGGAGCAACCTTg  <  1:1327495/62‑1 (MQ=255)
aGCATTCTCTGCAGGACGTTAAAGCATTGGGTATTGCCGGCCAGATGCACGGAGCAACCTTg  <  1:1031911/62‑1 (MQ=255)
aGCATTCTCTGCAGGACGTTAAAGCATTGGGTATTGCCGGCCAGATGCACGGAGCAACCTTg  <  1:840485/62‑1 (MQ=255)
aGCATTCTCTGCAGGACGTTAAAGCATTGGGTATTGCCGGCCAGATGCACGGAGCAACCTTg  <  1:946525/62‑1 (MQ=255)
aGCATTCTCTGCAGGACGTTAAAGCATTGGGTATTGCCGGCCAGATGCACGGAGCAACCTTg  <  1:701697/62‑1 (MQ=255)
aGCATTCTCTGCAGGACGTTAAAGCATTGGGTATTGCCGGCCAGATGCACGGAGCAACCTTg  <  1:1139508/62‑1 (MQ=255)
aGCATTCTCTGCAGGACGTAAAAGCATTGGGTATTGCCGGCCAGATGCACGGAGCAACCTTg  <  1:2204431/62‑1 (MQ=255)
 gCATTCTCTGCAGGACGTTAAAGCATTGGGTATTGCCGGCCAGATGCACGGAGCAACCTTg  <  1:2347103/61‑1 (MQ=255)
  cATTCTCTGCAGGACGTTAAAGCATTGGGTATTGCCGGCCAGATGCACGGAGCAACCTTg  <  1:345147/60‑1 (MQ=255)
     tctcTGCAGGACGTTAAAGCATTGGGTATTGCCGGCCAGATGCACGGAGCAACCTTg  <  1:1434299/57‑1 (MQ=255)
                      aGCATTGGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:2611793/1‑36 (MQ=25)
                      aGCATTAGGTATTGCGGGACAGATGCACGGTGCAAc      >  1:1440064/1‑36 (MQ=19)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCaa       >  1:1979921/1‑35 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:2770683/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:935232/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:2728067/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:930519/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:2724708/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:2709570/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:2708611/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:2705236/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:849245/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:2657278/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:2644509/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:2628023/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:2610330/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1002182/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:369411/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:612962/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:697183/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:570700/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:483636/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:461925/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:452615/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:447496/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:422791/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:788364/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:2809520/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:951123/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:330280/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:807234/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:2824468/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:842906/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:2811731/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:676547/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:869419/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1240489/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:2503119/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1529861/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1500024/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:139871/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:138789/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:13097/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1297454/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1251062/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1249850/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1588858/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1229337/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1221281/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1168442/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1159140/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1153254/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1120361/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1072356/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1040219/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1026126/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1956603/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:2497513/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:2495390/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:2454107/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:2349641/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:2203742/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:2123460/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:2109383/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:2039819/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:200124/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1611748/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1877668/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1864359/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1755210/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1726641/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1698703/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1665175/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1658715/1‑36 (MQ=25)
                      aGCATTAGGTATTGCCGGACAGATGCACGGTGCAAc      >  1:1612127/1‑36 (MQ=25)
                      aGCATTAGGTATTGACGGACAGATGCACGGTGCAAc      >  1:1509501/1‑36 (MQ=19)
                                        |                     
AGCATTCTCTGCAGGACGTTAAAGCATTGGGTATTGCCGGCCAGATGCACGGAGCAACCTTG  >  W3110S.gb/3911230‑3911291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑