Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,915,242	G→T	E48D (GAG→GAT)	glyQ →	glycine tRNA synthetase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,915,242	0	G	T	100.0%	17.5 / NA	7	E48D (GAG→GAT)	glyQ	glycine tRNA synthetase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (7/0);  total (7/0)

GAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTAT  >  W3110S.gb/3915211‑3915272                                |                               gAACCTCTCACCCAATGACCTGTCTGCGCGATCTGGGGCCAGAACCGATGGCGGCTGCTTAt  >  1:1547227/1‑62 (MQ=255) gAACCTCTCACCCAATGACCTGTCTGCGCGATCTGGGGCCAGAACCGATGGCGGCTGCTTAt  >  1:1691643/1‑62 (MQ=255) gAACCTCTCACCCAATGACCTGTCTGCGCGATCTGGGGCCAGAACCGATGGCGGCTGCTTAt  >  1:1910243/1‑62 (MQ=255) gAACCTCTCACCCAATGACCTGTCTGCGCGATCTGGGGCCAGAACCGATGGCGGCTGCTTAt  >  1:1917013/1‑62 (MQ=255) gAACCTCTCACCCAATGACCTGTCTGCGCGATCTGGGGCCAGAACCGATGGCGGCTGCTTAt  >  1:1969991/1‑62 (MQ=255) gAACCTCTCACCCAATGACCTGTCTGCGCGATCTGGGGCCAGAACCGATGGCGGCTGCTTAt  >  1:516946/1‑62 (MQ=255) gAACCTCTCACCCAATGACCTGTCTGCGCGATCTCGGGCCa                       >  1:1587350/1‑41 (MQ=38)                                |                               GAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTAT  >  W3110S.gb/3915211‑3915272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑