Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,964,846	C→A	T262T (ACG→ACT)	yhjE ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,964,846	0	C	A	81.6%	96.0 / 22.9	49	T262T (ACG→ACT)	yhjE	predicted transporter
Reads supporting (aligned to +/- strand):  ref base C (0/9);  new base A (0/40);  total (0/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

GTACTAAAGGTCATAGAGTAGACCGTCATGATGTAAAACAGCGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAC  >  W3110S.gb/3964805‑3964893                                          |                                                gTACTAAAGGTCATAGAGTAGACCGTCATGATGTAAAACAGCGTATAGGTTGCCAGCATAAt                             <  1:2530381/62‑1 (MQ=255) gTACTAAAGGTCATAGAGTAGACCGTCATGATGTAAAACAGCGTATAGGTTGCCAGCATAAt                             <  1:1164565/62‑1 (MQ=255) gTACTAAAGGTCATAGAGTAGACCGTCATGATGTAAAACAGCGTATAGGTTGCCAGCATAAt                             <  1:1428183/62‑1 (MQ=255) gTACTAAAGGTCATAGAGTAGACCGTCATGATGTAAAACAGCGTATAGGTTGCCAGCATAAt                             <  1:2245264/62‑1 (MQ=255) gTACTAAAGGTCATAGAGTAGACCGTCATGATGTAAAACAGCGTATAGGTTGCCAGCATAAt                             <  1:2762353/62‑1 (MQ=255) gTACTAAAGGTCATAGAGTAGACCGTCATGATGTAAAACAGCGTATAGGTTGCCAGCATAAt                             <  1:2021432/62‑1 (MQ=255)  tACTAAAGGTCATAGAGTAGACCGTCATGATGTAAAACAGCGTATAGGTTGCCAGCATAAt                             <  1:2759007/61‑1 (MQ=255)  tACTAAAGGTCATAGAGTAGACCGTCATGATGTAAAACAGCGTATAGGTTGCCAGCATAAt                             <  1:2157739/61‑1 (MQ=255)                            atgatgTAAAACAGCGTATAGGTTGCCAGCATAAt                             <  1:1510610/35‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:586037/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:246909/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:2538171/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:2653890/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:2878423/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:2889727/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:2915856/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:2314157/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:760254/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:791988/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:800214/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:839376/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:849790/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:86068/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:909609/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:921282/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:923190/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:964658/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:2404555/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:1181306/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:1220395/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:128021/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:1400000/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:1504910/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:1697816/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:1732992/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:1795830/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:1922641/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:1928434/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:1936024/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:197099/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:2130291/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:218316/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:2184655/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:221025/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:223671/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:2285794/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:2342319/62‑1 (MQ=255)                            atgatgTAAAACAGAGTATAAGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:624463/62‑1 (MQ=255)                            atgaagTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc  <  1:2453713/62‑1 (MQ=255)                                          |                                                GTACTAAAGGTCATAGAGTAGACCGTCATGATGTAAAACAGCGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAC  >  W3110S.gb/3964805‑3964893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑