| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,976,263 | G→A | L155L (CTG→CTA) | gadW → | DNA‑binding transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,976,263 | 0 | G | A | 85.7% | 233.5 / 27.3 | 98 | L155L (CTG→CTA) | gadW | DNA‑binding transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base G (0/14); new base A (0/84); total (0/98) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.13e-01 | |||||||||||
Only 100 of 107 total aligned reads displayed.
AAAGTTGAACGCCTTATTAGCTTTGATATCGCCAAACGTTGGTATCTGCGCGATATCGCGGAAAGAATGTATACCAGCGAGAGTCTAATCAAAAAAAAGTTGCAGGAT > W3110S.gb/3976216‑3976323 | aaaGTTGAACGCCTTATTAGCTTTGATATCGCCAAACGTTGGTATCTGCGCGATATCGCGGa < 1:338098/62‑1 (MQ=255)aaaGTTGAACGCCTTATTAGCTTTGATATCGCCAAACGTTGGTATCTGCGCGATATCGCGGa < 1:1008337/62‑1 (MQ=255)aaaGTTGAACGCCTTATTAGCTTTGATATCGCCAAACGTTGGTATCTGCGCGATATCGCGGa < 1:2641041/62‑1 (MQ=255)aaaGTTGAACGCCTTATTAGCTTTGATATCGCCAAACGTTGGTATCTGCGCGATATCGCGGa < 1:2729451/62‑1 (MQ=255)aaaGTTGAACGCCTTATTAGCTTTGATATCGCCAAACGTTGGTATCTGCGCGATATCGCGGa < 1:1954876/62‑1 (MQ=255)aaaGTTGAACGCCTTATTAGCTTTGATATCGCCAAACGTTGGTATCTGCGCGATATCGCGGa < 1:1910036/62‑1 (MQ=255)aaaGTTGAACGCCTTATTAGCTTTGATATCGCCAAACGTTGGTATCTGCGCGATATCGCGGa < 1:309925/62‑1 (MQ=255)aaaGTTGAACGCCTTATTAGCTTTGATATCGCCAAACGTTGGTATCTGCGCGATATCGCGGa < 1:1402033/62‑1 (MQ=255)aaaGTTGAACGCCTTATTAGCTTTGATATCGCCAAACGTTGGTATCTGCGCGATATCGCGGa < 1:1081106/62‑1 (MQ=255)aaaGTTGAACGCCTTATTAGCTTTGATATCGCCAAACGTTGGTATCTGCGCGATATCGCGGa < 1:95392/62‑1 (MQ=255)aaaGTTGAACGCCTTATTAGCTTTGATATCGCCAAACGTTGGTATCTGCGCGATATCGCGGa < 1:905399/62‑1 (MQ=255)aaaGTTGAACGCCTTATTAGCTTTGATATCGCCAAACGTTGGTATCTGCGCGATATCGCGGa < 1:865646/62‑1 (MQ=255)aaaGTTGAACGCCTTATTAGCTTTGATATCGCCAAACGTTGGTATCTGCGCGATATCGCGGa < 1:833225/62‑1 (MQ=255)aaaGTTGAACGCCTTATTAACTTTGATATCGCCAAACGTTGGTATCTGCGCGATATCGCGGa < 1:1190631/62‑1 (MQ=255) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2779898/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2807583/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2775204/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2766636/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2762373/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2747078/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:274567/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2736760/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:654956/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2712323/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2689851/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:94911/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2630395/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2623188/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2617670/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2598528/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2566831/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2516849/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:426496/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:595449/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:592492/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:638115/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:566894/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:533653/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:531148/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:464561/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:452393/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:437640/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:61494/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:371786/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2392662/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:337245/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:854327/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2898223/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:791188/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2895700/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:639427/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:1231416/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:1801576/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:1698017/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2445907/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:1594761/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:1543135/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:14851/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:1460695/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:1432943/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:1358144/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:1329676/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:1652764/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:1222529/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:1220394/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:1169384/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:1144004/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:1111865/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:1068842/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:1065384/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:1056376/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:1018685/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2270803/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2094391/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2088056/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2110141/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2083579/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2083429/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2031969/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2239216/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2000288/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2250470/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:1988958/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:1980888/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:1958577/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2280394/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:230399/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:1956164/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:1944786/42‑1 (MQ=38) attaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:1839338/42‑1 (MQ=38) ttaGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATc < 1:2380145/41‑1 (MQ=38) gcgcGATATCGCGGAAAGAATGTATACCAGCGAGAGTCTAATCAAAAATAAGTTGCAGGAt > 1:2090142/1‑61 (MQ=255) gcgcGATATCGCGGAAAGAATGTATACCAGCGAGAGTCTAATCAAAAAAAAGTTGCAGGAt > 1:1249836/1‑61 (MQ=255) gcgcGATATCGCGGAAAGAATGTATACCAGCGAGAGTCTAATCAAAAAAAAGTTGCAGGAt > 1:1459163/1‑61 (MQ=255) gcgcGATATCGCGGAAAGAATGTATACCAGCGAGAGTCTAATCAAAAAAAAGTTGCAGGAt > 1:498196/1‑61 (MQ=255) gcgcGATATCGCGGAAAGAATGTATACCAGCGAGAGTCTAATCAAAAAAAAGTTGCAGGAt > 1:856459/1‑61 (MQ=255) gcgcGATATCGCGGAAAGAATGTATACCAGCGAGAGTCTAATCAAAAAAAAGTTGCAGGAt > 1:473983/1‑61 (MQ=255) gcgcGATATCGCGGAAAGAATGTATACCAGCGAGAGTCTAATCAAAAAAAAGTTGCAGGAt > 1:433928/1‑61 (MQ=255) gcgcGATATCGCGGAAAGAATGTATACCAGCGAGAGTCTAATCAAAAAAAAGTTGCAGGAt > 1:1604869/1‑61 (MQ=255) gcgcGATATCGCGGAAAGAATGTATACCAGCGAGAGTCTAATCAAAAAAAAGTTGCAGGAt > 1:1740566/1‑61 (MQ=255) | AAAGTTGAACGCCTTATTAGCTTTGATATCGCCAAACGTTGGTATCTGCGCGATATCGCGGAAAGAATGTATACCAGCGAGAGTCTAATCAAAAAAAAGTTGCAGGAT > W3110S.gb/3976216‑3976323 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |