Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,013,511	C→T	A878V (GCG→GTG)	rbbA →	fused ribosome‑associated ATPases

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,013,511	0	C	T	100.0%	170.2 / NA	52	A878V (GCG→GTG)	rbbA	fused ribosome‑associated ATPases
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/28);  total (24/28)

CTGACTATCGCCCGCGGGACGTTCTCGAAAGCGCTGGATCTGACTGATTTGTGGCAACTTTTTATCC  >  W3110S.gb/4013480‑4013546                                |                                    ctaaCTATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACtttt       >  1:1892043/4‑62 (MQ=255) cTGACTATCGCCCGCGGGACGTTCTCGAAAGTGCTGGa                               >  1:2225270/1‑38 (MQ=255) cTGACTATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACtttt       >  1:917871/1‑62 (MQ=255) cTGACTATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACtttt       >  1:1011440/1‑62 (MQ=255) cTGACTATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACtttt       >  1:520701/1‑62 (MQ=255) cTGACTATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACtttt       >  1:466336/1‑62 (MQ=255) cTGACTATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACtttt       >  1:2831872/1‑62 (MQ=255) cTGACTATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACtttt       >  1:2580375/1‑62 (MQ=255) cTGACTATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACtttt       >  1:250705/1‑62 (MQ=255) cTGACTATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACtttt       >  1:2441305/1‑62 (MQ=255) cTGACTATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACtttt       >  1:2376393/1‑62 (MQ=255) cTGACTATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACtttt       >  1:222991/1‑62 (MQ=255) cTGACTATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACtttt       >  1:2202908/1‑62 (MQ=255) cTGACTATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACtttt       >  1:2188184/1‑62 (MQ=255) cTGACTATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACtttt       >  1:1968145/1‑62 (MQ=255) cTGACTATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACtttt       >  1:1195251/1‑62 (MQ=255) cTGACTATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACtttt       >  1:1740364/1‑62 (MQ=255) cTGACTATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACtttt       >  1:1799710/1‑62 (MQ=255) cTGACTATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACtttt       >  1:1403698/1‑62 (MQ=255) cTGACTATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACtttt       >  1:1279299/1‑62 (MQ=255) cTGACTATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACtttt       >  1:1223833/1‑62 (MQ=255) cTGACTATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACtttt       >  1:1219007/1‑62 (MQ=255) cTGACTATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACttt        >  1:2363065/1‑61 (MQ=255) cTGACTATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTCATTTGTGGCAACttt        >  1:2555272/1‑61 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGAGTGATTTGTGGCAACTTTTTATcc  <  1:2604454/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:587770/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:583070/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:634989/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:694757/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:809523/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:2609906/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:83411/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:895750/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:905103/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:2585028/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:1397064/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:1349296/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:1381952/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:2371688/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:2330996/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:22780/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:1507356/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:1563940/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:2212371/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:2204734/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:1638819/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:1654722/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:2146437/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:1719730/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:1904413/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:1898816/62‑1 (MQ=255)      tATCGCCCGCGGGACGTTCTCGAAAGTGCTGGATCTGACTGATTTGTGGCAACTTTTTATcc  <  1:1830895/62‑1 (MQ=255)                                |                                    CTGACTATCGCCCGCGGGACGTTCTCGAAAGCGCTGGATCTGACTGATTTGTGGCAACTTTTTATCC  >  W3110S.gb/4013480‑4013546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑