Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,134,096	C→T	L339F (CTT→TTT)	yhfT →	predicted inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,134,096	0	C	T	100.0%	29.8 / NA	11	L339F (CTT→TTT)	yhfT	predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/6);  total (5/6)

CGGCGGAAGTCTTGCTGCTTCGTTCGATCGGCAAATGGCTGGGACGCTACCCGTCGGTGCGTAATGC  >  W3110S.gb/4134079‑4134145                  |                                                  cggcggAAGTCTTGCTGTTTCGTTCGCTCGGCAAATGGCTGGGACGCTACCCGTCGGTGCg        >  1:2859363/1‑61 (MQ=255) cggcggAAGTCTTGCTGTTTCGTTCGATCGGCAAATGGCTGGGACGCTACCCGTCGGTGCGt       >  1:1643296/1‑62 (MQ=255) cggcggAAGTCTTGCTGTTTCGTTCGATCGGCAAATGGCTGGGACGCTACCCGTCGGTGCGt       >  1:1653570/1‑62 (MQ=255) cggcggAAGTCTTGCTGTTTCGTTCGATCGGCAAATGGCTGGGACGCTACCCGTCGGTGCGt       >  1:708306/1‑62 (MQ=255) cggcggAAGTCTTGCTGTTTCGTTCGATCGGCAAATGGCTGGGACGCTACCCGTCGGTGCGt       >  1:786391/1‑62 (MQ=255)      gAAGTCTTGCTGTTTCGTTCGATCGGCAAATGGCTGGGACGCTACCCGTCGGTGCGTAATGc  <  1:1404768/62‑1 (MQ=255)      gAAGTCTTGCTGTTTCGTTCGATCGGCAAATGGCTGGGACGCTACCCGTCGGTGCGTAATGc  <  1:1941956/62‑1 (MQ=255)      gAAGTCTTGCTGTTTCGTTCGATCGGCAAATGGCTGGGACGCTACCCGTCGGTGCGTAATGc  <  1:2263356/62‑1 (MQ=255)      gAAGTCTTGCTGTTTCGTTCGATCGGCAAATGGCTGGGACGCTACCCGTCGGTGCGTAATGc  <  1:306251/62‑1 (MQ=255)      gAAGTCTTGCTGTTTCGTTCGATCGGCAAATGGCTGGGACGCTACCCGTCGGTGCGTAATGc  <  1:595443/62‑1 (MQ=255)      gAAGTCTTGCTGTTTCGTTCGATCGGCAAATGGCTGGGACGCTACCCGTCGGTGCGTAATGc  <  1:673078/62‑1 (MQ=255)                  |                                                  CGGCGGAAGTCTTGCTGCTTCGTTCGATCGGCAAATGGCTGGGACGCTACCCGTCGGTGCGTAATGC  >  W3110S.gb/4134079‑4134145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑