| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 4,175,619 | C→T | R293H (CGC→CAC) | gspL ← | general secretory pathway component, cryptic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,175,619 | 0 | C | T | 85.7% | 10.5 / ‑3.5 | 7 | R293H (CGC→CAC) | gspL | general secretory pathway component, cryptic |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (0/6); total (0/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACAGCTTCAGGATATTGCTGAGCGAGTTGCTGCTTAAAGTAAAAACGAAAATTGTGGGTGCGTTTGATCTGCGGGAAATAACG > W3110S.gb/4175558‑4175641 | cacaGCTTCAGGATATTGCTGAGCGAGTTGCTGCTTAAAGTAAAAACGAAAATTGTGGGTGc > 1:1881730/1‑62 (MQ=255)cacaGCTTCAGGATATTGCTGAGCGAGTTGCTGCTTAAAGTAAAAACGAAAATTGTGGGTGc > 1:922740/1‑62 (MQ=255)cacaGCTTCAGGATATTGCTGAGCGAGTTGCTGCTTAAAGTAAAAACGAAAATTGTGGGTGc > 1:896641/1‑62 (MQ=255)cacaGCTTCAGGATATTGCTGAGCGAGTTGCTGCTTAAAGTAAAAACGAAAATTGTGGGTGc > 1:67937/1‑62 (MQ=255)cacaGCTTCAGGATATTGCTGAGCGAGTTGCTGCTTAAAGTAAAAACGAAAATTGTGGGTGc > 1:628278/1‑62 (MQ=255)cacaGCTTCAGGATATTGCTGAGCGAGTTGCTGCTTAAAGTAAAAACGAAAATTGTGGGTGc > 1:47769/1‑62 (MQ=255)cacaGCTTCAGGATATTGCTGAGCGAGTTGCTGCTTAAAGTAAAAACGAAAATTGTGGGTGc > 1:318644/1‑62 (MQ=255)cacaGCTTCAGGATATTGCTGAGCGAGTTGCTGCTTAAAGTAAAAACGAAAATTGTGGGTGc > 1:2925741/1‑62 (MQ=255)cacaGCTTCAGGATATTGCTGAGCGAGTTGCTGCTTAAAGTAAAAACGAAAATTGTGGGTGc > 1:2718344/1‑62 (MQ=255)cacaGCTTCAGGATATTGCTGAGCGAGTTGCTGCTTAAAGTAAAAACGAAAATTGTGGGTGc > 1:2652741/1‑62 (MQ=255)cacaGCTTCAGGATATTGCTGAGCGAGTTGCTGCTTAAAGTAAAAACGAAAATTGTGGGTGc > 1:2336619/1‑62 (MQ=255)cacaGCTTCAGGATATTGCTGAGCGAGTTGCTGCTTAAAGTAAAAACGAAAATTGTGGGTGc > 1:2319223/1‑62 (MQ=255)cacaGCTTCAGGATATTGCTGAGCGAGTTGCTGCTTAAAGTAAAAACGAAAATTGTGGGTGc > 1:1028686/1‑62 (MQ=255)cacaGCTTCAGGATATTGCTGAGCGAGTTGCTGCTTAAAGTAAAAACGAAAATTGTGGGTGc > 1:1602049/1‑62 (MQ=255)cacaGCTTCAGGATATTGCTGAGCGAGTTGCTGCTTAAAGTAAAAACGAAAATTGTGGGTGc > 1:1483010/1‑62 (MQ=255)cacaGCTTCAGGATATTGCTGAGCGAGTTGCTGCTTAAAGTAAAAACGAAAATTGTGGGTGc > 1:1324840/1‑62 (MQ=255)cacaGCTTCAGGATATTGCTGAGCGAGTTGCTGCTTAAAGTAAAAACGAAAATTGTGGGTGc > 1:1245490/1‑62 (MQ=255)cacaGCTTCAGGATATTGCTGAGCGAGTTGCTGCTTAAAGTAAAAACGAAAATTGTGGGTGc > 1:1206505/1‑62 (MQ=255)cacaGCTTCAGGATATTGCTGAGCGAGTTGCTGCTTAAAGTAAAAACGAAAATTGTGGGTGc > 1:1129803/1‑62 (MQ=255)cacaGCTTCAGGATATTGCTGAGCGAGTTGCTGCTTAAAGTAAAAACGAAAATTGTGGGTGc > 1:1114401/1‑62 (MQ=255) atTGCTGAGCGAGTTGCTGCTTAAAGTAAAAACGAAAATTGTGGGTGCGTTTGATCTGCggg < 1:1311596/62‑1 (MQ=255) ctTAAAGTAAAAACGAAAGTTGTGGGTGTGTTTGATCTGCGGGAAATAACg < 1:1716775/51‑1 (MQ=39) ctTAAAGTAAAAACGAAAGTTGTGGGTGTGTTTGATCTGCGGGAAATAACg < 1:2164297/51‑1 (MQ=39) ctTAAAGTAAAAACGAAAGTTGTGGGTGTGTTTGATCTGCGGGAAATAACg < 1:1275506/51‑1 (MQ=39) ctTAAAGTAAAAACGAAAGTTGTGGGTGTGTTTGATCTGCGGGAAATAACg < 1:1262487/51‑1 (MQ=39) ctTAAAGTAAAAACGAAAGTTGTGGGTGTGTTTGATCTGCGGGAAATAACg < 1:1247674/51‑1 (MQ=39) aaCGAAAGTTGTGGGTGTGTTTGATCTGCGGGAAATAACg < 1:2577436/40‑1 (MQ=37) | CACAGCTTCAGGATATTGCTGAGCGAGTTGCTGCTTAAAGTAAAAACGAAAATTGTGGGTGCGTTTGATCTGCGGGAAATAACG > W3110S.gb/4175558‑4175641 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |