Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,178,794	A→T	L53I (TTA→ATA)	gspH ←	predicted general secretory pathway component, cryptic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,178,794	0	A	T	100.0%	41.3 / NA	15	L53I (TTA→ATA)	gspH	predicted general secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (0/15);  total (0/15)

GACCACTGAGCGTGGCGCGGTCGATGGCTAATTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACATCCTC  >  W3110S.gb/4178764‑4178854                               |                                                             gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt                               <  1:973046/62‑1 (MQ=255) gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt                               <  1:428191/62‑1 (MQ=255) gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt                               <  1:2788995/62‑1 (MQ=255) gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt                               <  1:2734371/62‑1 (MQ=255) gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt                               <  1:2695188/62‑1 (MQ=255) gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt                               <  1:615140/62‑1 (MQ=255) gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt                               <  1:2413682/62‑1 (MQ=255) gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt                               <  1:2294163/62‑1 (MQ=255) gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt                               <  1:2047777/62‑1 (MQ=255) gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt                               <  1:1923308/62‑1 (MQ=255) gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt                               <  1:1631055/62‑1 (MQ=255) gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt                               <  1:1487782/62‑1 (MQ=255) gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt                               <  1:1356224/62‑1 (MQ=255)  aCCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCTGTAAACCGCGCCGCTGtt                               <  1:2523727/61‑1 (MQ=255)  aCCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt                               <  1:171319/61‑1 (MQ=255)                              aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc  <  1:953678/62‑1 (MQ=255)                              aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc  <  1:924024/62‑1 (MQ=255)                              aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc  <  1:693301/62‑1 (MQ=255)                              aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc  <  1:963220/62‑1 (MQ=255)                              aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc  <  1:2644212/62‑1 (MQ=255)                              aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc  <  1:591127/62‑1 (MQ=255)                              aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc  <  1:510479/62‑1 (MQ=255)                              aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc  <  1:445016/62‑1 (MQ=255)                              aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc  <  1:125294/62‑1 (MQ=255)                              aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc  <  1:2331372/62‑1 (MQ=255)                              aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc  <  1:2277566/62‑1 (MQ=255)                              aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc  <  1:1938574/62‑1 (MQ=255)                              aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc  <  1:1544999/62‑1 (MQ=255)                              aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc  <  1:1262498/62‑1 (MQ=255)                              aaTTCAAGTGCAGCGGTAAAACGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc  <  1:475741/62‑1 (MQ=255)                               |                                                             GACCACTGAGCGTGGCGCGGTCGATGGCTAATTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACATCCTC  >  W3110S.gb/4178764‑4178854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑