Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,270,333	T→C	D320D (GAT→GAC)	alr →	alanine racemase 1, PLP‑binding, biosynthetic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,270,333	0	T	C	100.0%	39.1 / NA	13	D320D (GAT→GAC)	alr	alanine racemase 1, PLP‑binding, biosynthetic
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/0);  total (13/0)

ATATGATCTGCGTAGACTTAGGTCCACAGGCGCAGGACAAAGCCGGGGATCCGGTCATTTTA  >  W3110S.gb/4270284‑4270345                                                  |             atatGATCTGCGTAGACTTAGGTCCACAGGCGCAGGACAAAGCCGGGGACCCGGTCATTTTa  >  1:1047602/1‑62 (MQ=255) atatGATCTGCGTAGACTTAGGTCCACAGGCGCAGGACAAAGCCGGGGACCCGGTCATTTTa  >  1:1274368/1‑62 (MQ=255) atatGATCTGCGTAGACTTAGGTCCACAGGCGCAGGACAAAGCCGGGGACCCGGTCATTTTa  >  1:1572377/1‑62 (MQ=255) atatGATCTGCGTAGACTTAGGTCCACAGGCGCAGGACAAAGCCGGGGACCCGGTCATTTTa  >  1:1606490/1‑62 (MQ=255) atatGATCTGCGTAGACTTAGGTCCACAGGCGCAGGACAAAGCCGGGGACCCGGTCATTTTa  >  1:1846275/1‑62 (MQ=255) atatGATCTGCGTAGACTTAGGTCCACAGGCGCAGGACAAAGCCGGGGACCCGGTCATTTTa  >  1:1876680/1‑62 (MQ=255) atatGATCTGCGTAGACTTAGGTCCACAGGCGCAGGACAAAGCCGGGGACCCGGTCATTTTa  >  1:1892329/1‑62 (MQ=255) atatGATCTGCGTAGACTTAGGTCCACAGGCGCAGGACAAAGCCGGGGACCCGGTCATTTTa  >  1:2211320/1‑62 (MQ=255) atatGATCTGCGTAGACTTAGGTCCACAGGCGCAGGACAAAGCCGGGGACCCGGTCATTTTa  >  1:307731/1‑62 (MQ=255) atatGATCTGCGTAGACTTAGGTCCACAGGCGCAGGACAAAGCCGGGGACCCGGTCATTTTa  >  1:500854/1‑62 (MQ=255) atatGATCTGCGTAGACTTAGGTCCACAGGCGCAGGACAAAGCCGGGGACCCGGTCATTTTa  >  1:724699/1‑62 (MQ=255) atatGATCTGCGTAGACTTAGGTCCACAGGCGCAGGACAAAGCCGGGGACCCGGTCATTTTa  >  1:971507/1‑62 (MQ=255) atatGATCTGCGTAGACTTAGGTCCACAGGCGCAGGACAAAGCCGGGGACCCGGTCATTTTa  >  1:991987/1‑62 (MQ=255)                                                  |             ATATGATCTGCGTAGACTTAGGTCCACAGGCGCAGGACAAAGCCGGGGATCCGGTCATTTTA  >  W3110S.gb/4270284‑4270345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑