Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,336,183	C→A	A334A (GCC→GCA)	proP →	proline/glycine betaine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,336,183	0	C	A	100.0%	94.5 / NA	31	A334A (GCC→GCA)	proP	proline/glycine betaine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (31/0);  total (31/0)

GACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATT  >  W3110S.gb/4336139‑4336243                                             |                                                             gACCGTTTTGGCCGTCGTCCGTTTGTGGTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:2811516/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGa                                              >  1:882759/1‑61 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:2322034/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:982106/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:937577/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:777590/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:75690/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:714497/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:714270/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:684119/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:669844/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:455487/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:441054/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:2845133/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:2506156/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:2375669/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:1917705/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:1107609/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:1229695/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:1405546/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:1440308/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:1595018/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:1702410/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:1062228/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:2096/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:2226927/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:223250/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:2256767/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:2271683/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTTTGTGTTGGCGAt                                             >  1:2281446/1‑62 (MQ=255) gACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTACACTGTTTGTGTTGGCGAt                                             >  1:1899051/1‑62 (MQ=255)                                            cccTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGAtt  >  1:301524/1‑62 (MQ=255)                                            cccTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGAtt  >  1:1580903/1‑62 (MQ=255)                                             |                                                             GACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATT  >  W3110S.gb/4336139‑4336243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑