| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 4,342,084 | G→T | P176T (CCA→ACA) | adiY ← | DNA‑binding transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,342,084 | 0 | G | T | 76.0% | 29.6 / 14.2 | 25 | P176T (CCA→ACA) | adiY | DNA‑binding transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base G (0/6); new base T (0/19); total (0/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.32e-01 | |||||||||||
CTTTTTCTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCT > W3110S.gb/4342064‑4342127 | cTTTTTCTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGt < 1:944227/62‑1 (MQ=255)cTTTTTCTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGt < 1:385756/62‑1 (MQ=255)cTTTTTCTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGt < 1:2810447/62‑1 (MQ=255)cTTTTTCTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGt < 1:2023290/62‑1 (MQ=255)cTTTTTCTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGt < 1:2141950/62‑1 (MQ=255)cTTTTTCTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGt < 1:2153029/62‑1 (MQ=255) ttttCTTTAACAGACGTGTGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCt < 1:2404987/62‑1 (MQ=255) ttttCTTTAACAGACGTGTGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCt < 1:825404/62‑1 (MQ=255) ttttCTTTAACAGACGTGTGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCt < 1:4704/62‑1 (MQ=255) ttttCTTTAACAGACGTGTGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCt < 1:353317/62‑1 (MQ=255) ttttCTTTAACAGACGTGTGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCt < 1:2806218/62‑1 (MQ=255) ttttCTTTAACAGACGTGTGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCt < 1:2770777/62‑1 (MQ=255) ttttCTTTAACAGACGTGTGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCt < 1:2769139/62‑1 (MQ=255) ttttCTTTAACAGACGTGTGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCt < 1:2609522/62‑1 (MQ=255) ttttCTTTAACAGACGTGTGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCt < 1:2498404/62‑1 (MQ=255) ttttCTTTAACAGACGTGTGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCt < 1:2493684/62‑1 (MQ=255) ttttCTTTAACAGACGTGTGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCt < 1:1017640/62‑1 (MQ=255) ttttCTTTAACAGACGTGTGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCt < 1:1780888/62‑1 (MQ=255) ttttCTTTAACAGACGTGTGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCt < 1:1630011/62‑1 (MQ=255) ttttCTTTAACAGACGTGTGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCt < 1:1464858/62‑1 (MQ=255) ttttCTTTAACAGACGTGTGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCt < 1:1395639/62‑1 (MQ=255) ttttCTTTAACAGACGTGTGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCt < 1:1263096/62‑1 (MQ=255) ttttCTTTAACAGACGTGTGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCt < 1:1077021/62‑1 (MQ=255) tttCTTTAACAGACGTGTGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCt < 1:2394213/61‑1 (MQ=255) aGACGTGTGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCt < 1:1174687/51‑1 (MQ=255) | CTTTTTCTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCT > W3110S.gb/4342064‑4342127 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |