Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,348,538	C→G	L160V (CTA→GTA)	melB →	melibiose:sodium symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,348,538	0	C	G	100.0%	32.2 / NA	11	L160V (CTA→GTA)	melB	melibiose:sodium symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (0/11);  total (0/11)

CCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTG  >  W3110S.gb/4348484‑4348580                                                       |                                           ccTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGGTACCAtt                                     <  1:2796976/62‑1 (MQ=255)                                      ttACGGCAGGTGTGACGGTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTg  <  1:116566/60‑1 (MQ=255)                                      ttACGGCAGGTGTGACGGTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTg  <  1:1293639/60‑1 (MQ=255)                                      ttACGGCAGGTGTGACGGTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTg  <  1:1463954/60‑1 (MQ=255)                                      ttACGGCAGGTGTGACGGTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTg  <  1:1638520/60‑1 (MQ=255)                                      ttACGGCAGGTGTGACGGTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTg  <  1:1691027/60‑1 (MQ=255)                                      ttACGGCAGGTGTGACGGTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTg  <  1:2499997/60‑1 (MQ=255)                                      ttACGGCAGGTGTGACGGTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTg  <  1:2595471/60‑1 (MQ=255)                                      ttACGGCAGGTGTGACGGTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTg  <  1:569601/60‑1 (MQ=255)                                       tACGGCAGGTGTGACGGTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTg  <  1:1516710/59‑1 (MQ=255)                                                  tgACGGTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTg  <  1:2801042/48‑1 (MQ=255)                                                       |                                           CCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTG  >  W3110S.gb/4348484‑4348580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑