Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,373,597	G→A	L84L (CTG→CTA)	fxsA →	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,373,597	0	G	A	100.0%	57.7 / NA	19	L84L (CTG→CTA)	fxsA	inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/10);  total (9/10)

CAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTACCGGGCTTTTTTACCGACTTC  >  W3110S.gb/4373548‑4373627                                                  |                               cAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTACTTTTATTACCg                    >  1:908954/1‑62 (MQ=255) cAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTACTTTTATTACCg                    >  1:313008/1‑62 (MQ=255) cAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTACTTTTATTACCg                    >  1:1665856/1‑62 (MQ=255) cAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTACTTTTATTACCg                    >  1:2920290/1‑62 (MQ=255) cAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTACTTTTATTACCg                    >  1:2888565/1‑62 (MQ=255) cAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTACTTTTATTACCg                    >  1:2092603/1‑62 (MQ=255) cAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTACTTTTATTACCg                    >  1:2122146/1‑62 (MQ=255) cAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTACTTTTATTACCg                    >  1:2306100/1‑62 (MQ=255) cAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTACTTTTATTACCg                    >  1:2530835/1‑62 (MQ=255)                    taGTGTTTCGCTGATCATTGCTGGTTTGCTACTTTTATTACCGGGCTTTTTTACCGACTTc  <  1:2723131/60‑1 (MQ=255)                    aaGTGTTTCGCTGATCATTGCTGGTTTGCTACTTTTATTACCGGGCTTTTTTACCGACTTc  <  1:919134/61‑1 (MQ=255)                    aaGTGTTTCGCTGATCATTGCTGGTTTGCTACTTTTATTACCGGGCTTTTTTACCGACTTc  <  1:1239968/61‑1 (MQ=255)                    aaGTGTTTCGCTGATCATTGCTGGTTTGCTACTTTTATTACCGGGCTTTTTTACCGACTTc  <  1:361595/61‑1 (MQ=255)                    aaGTGTTTCGCTGATCATTGCTGGTTTGCTACTTTTATTACCGGGCTTTTTTACCGACTTc  <  1:1945142/61‑1 (MQ=255)                    aaGTGTTTCGCTGATCATTGCTGGTTTGCTACTTTTATTACCGGGCTTTTTTACCGACTTc  <  1:1723851/61‑1 (MQ=255)                    aaGTGTTTCGCTGATCATTGCTGGTTTGCTACTTTTATTACCGGGCTTTTTTACCGACTTc  <  1:1588319/61‑1 (MQ=255)                    aaGTGTTTCGCTGATCATTGCTGGTTTGCTACTTTTATTACCGGGCTTTTTTACCGACTTc  <  1:1530054/61‑1 (MQ=255)                    aaGTGTTTCGCTGATCATTGCTGGTTTGCTACTTTTATTACCGGGCTTTTTTACCGACTTc  <  1:1481497/61‑1 (MQ=255)                    aaGTGTTTCGCTGATCATTGCTGGTTTGCTACTTTTATTACCGGGCTTTTTTACCGACTTc  <  1:1314158/61‑1 (MQ=255)                                                  |                               CAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTACCGGGCTTTTTTACCGACTTC  >  W3110S.gb/4373548‑4373627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑