Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,418,573	C→T	G288G (GGC→GGT)	yjfC →	predicted synthetase/amidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,418,573	0	C	T	100.0%	66.5 / NA	21	G288G (GGC→GGT)	yjfC	predicted synthetase/amidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/0);  total (21/0)

CCTGGTTCGATGGCGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATC  >  W3110S.gb/4418560‑4418621              |                                                 ccTGTTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc  >  1:580311/1‑62 (MQ=255) ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCg            >  1:2292620/1‑52 (MQ=255) ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAAt   >  1:226255/1‑61 (MQ=255) ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc  >  1:2416325/1‑62 (MQ=255) ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc  >  1:986201/1‑62 (MQ=255) ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc  >  1:955417/1‑62 (MQ=255) ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc  >  1:888186/1‑62 (MQ=255) ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc  >  1:775958/1‑62 (MQ=255) ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc  >  1:525044/1‑62 (MQ=255) ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc  >  1:463365/1‑62 (MQ=255) ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc  >  1:2874580/1‑62 (MQ=255) ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc  >  1:2762461/1‑62 (MQ=255) ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc  >  1:2714434/1‑62 (MQ=255) ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc  >  1:120221/1‑62 (MQ=255) ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc  >  1:2341217/1‑62 (MQ=255) ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc  >  1:2138497/1‑62 (MQ=255) ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc  >  1:1871733/1‑62 (MQ=255) ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc  >  1:1743097/1‑62 (MQ=255) ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc  >  1:1727775/1‑62 (MQ=255) ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc  >  1:1593701/1‑62 (MQ=255) ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc  >  1:1421097/1‑62 (MQ=255)              |                                                 CCTGGTTCGATGGCGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATC  >  W3110S.gb/4418560‑4418621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑