Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,467,333	G→A	H4Y (CAT→TAT)	nrdD ←	anaerobic ribonucleoside‑triphosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,467,333	0	G	A	100.0%	166.2 / NA	50	H4Y (CAT→TAT)	nrdD	anaerobic ribonucleoside‑triphosphate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/28);  total (22/28)

GCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATGCGGTGTCATGATCGCTCCATATTTTTAAGAACAGGTTATCCACAGAAATTGG  >  W3110S.gb/4467275‑4467385                                                           |                                                     gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:396367/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:1876766/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:2086394/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:2308734/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:2710014/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:2762894/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:2827498/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:2881724/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:290929/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:1044120/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:55882/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:599430/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:74595/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:747458/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:87587/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:951252/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:952134/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:954717/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:1610646/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:1029830/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:1077146/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:1168321/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:1300187/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:1494237/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:1635120/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:1674205/62‑1 (MQ=255) gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:1724882/62‑1 (MQ=255)                    aTTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg                                                   <  1:1067697/43‑1 (MQ=255)                        aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGgtgt                                                >  1:607029/1‑42 (MQ=255)                        aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAttt                              >  1:1511910/1‑60 (MQ=255)                        aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAttt                              >  1:708824/1‑60 (MQ=255)                        aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAttt                              >  1:691640/1‑60 (MQ=255)                        aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAttt                              >  1:1934448/1‑60 (MQ=255)                        aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAttt                              >  1:1446546/1‑60 (MQ=255)                        aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAttt                              >  1:513938/1‑60 (MQ=255)                        aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAttt                              >  1:1150174/1‑60 (MQ=255)                        aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAttt                              >  1:2736689/1‑60 (MQ=255)                        aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAttt                              >  1:2234073/1‑60 (MQ=255)                        aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAttt                              >  1:1726174/1‑60 (MQ=255)                        aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAttt                              >  1:2450647/1‑60 (MQ=255)                        aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAttt                              >  1:1722916/1‑60 (MQ=255)                        aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAtt                               >  1:1508667/1‑59 (MQ=255)                        aaaCCGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATg                                            >  1:2150902/1‑46 (MQ=255)                                                    atcaCATACGGTGTCATGATCGCTCCATATTTTTAAGAACAGGTTATCCACAGAAATTgg  >  1:1809788/1‑60 (MQ=255)                                                    atcaCATACGGTGTCATGATCGCTCCATATTTTTAAGAACAGGTTATCCACAGAAATTgg  >  1:1177998/1‑60 (MQ=255)                                                    atcaCATACGGTGTCATGATCGCTCCATATTTTTAAGAACAGGTTATCCACAGAAATTgg  >  1:1968664/1‑60 (MQ=255)                                                    atcaCATACGGTGTCATGATCGCTCCATATTTTTAAGAACAGGTTATCCACAGAAATTgg  >  1:1764600/1‑60 (MQ=255)                                                    atcaCATACGGTGTCATGATCGCTCCATATTTTTAAGAACAGGTTATCCACAGAAATTgg  >  1:2604117/1‑60 (MQ=255)                                                    atcaCATACGGTGTCATGATCGCTCCATATTTTTAAGAACAGGTTATCCACAGAAATTgg  >  1:2458022/1‑60 (MQ=255)                                                    atcaCATACGGTGTCATGATCGCTCCATATTTTTAAGAACAGGTTATCCACAGAAATTgg  >  1:2375190/1‑60 (MQ=255)                                                           |                                                     GCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATGCGGTGTCATGATCGCTCCATATTTTTAAGAACAGGTTATCCACAGAAATTGG  >  W3110S.gb/4467275‑4467385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑