| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 4,489,152 | T→C | L494L (TTA→TTG) | pepA ← | aminopeptidase A, a cyteinylglycinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,489,152 | 0 | T | C | 87.4% | 244.6 / 21.0 | 95 | L494L (TTA→TTG) | pepA | aminopeptidase A, a cyteinylglycinase |
| Reads supporting (aligned to +/- strand): ref base T (11/1); new base C (0/83); total (11/84) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.54e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
CGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTG > W3110S.gb/4489095‑4489191 | cGGCAATAACAGCCTTGCCTGACGC‑ATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2431455/62‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCTCCGTTAAACCCAGCGCGGTTCAACAg < 1:1523657/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAg < 1:1692872/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2449226/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2810406/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2798770/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2794823/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:279076/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2662605/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2637160/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2559324/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2509151/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:245055/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2894867/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:239536/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2372297/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2312408/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2298500/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2291664/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2259208/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2227072/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2187814/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2147665/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:760184/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:95712/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:955389/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:882697/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:866516/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:856125/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:827584/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:810303/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:804861/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:790783/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:776178/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2882901/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:682555/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:570048/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:474496/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:451845/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:421488/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:367860/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:3451/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:335923/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2922632/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2153852/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:1353332/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:1678003/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:1676485/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:1671267/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:1579208/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:1554164/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:1510039/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:148679/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:147700/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:1454217/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:1578785/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:1309108/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:1288819/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:1266166/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:1235613/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:1132755/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:11194/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:1028128/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:1026289/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:101266/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2130503/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2128947/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2120708/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2066234/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2063175/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2033434/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2023839/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:1686652/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:1970070/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:1949386/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:1795436/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:1789401/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:1777553/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2133876/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGATTCAACAg < 1:324317/61‑1 (MQ=255) gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCATTAAACCCAGCGCGGTTCAACAg < 1:1890575/61‑1 (MQ=255) cAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:1896425/60‑1 (MQ=255) cTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:512987/50‑1 (MQ=255) aaTTACTCTTCGCCGTTAAACCCAGCGCGGTTCAACAg < 1:2051195/38‑1 (MQ=255) cGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTg > 1:137289/1‑62 (MQ=255) cGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTg > 1:1043799/1‑62 (MQ=255) cGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTg > 1:1107214/1‑62 (MQ=255) cGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTg > 1:899700/1‑62 (MQ=255) cGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTg > 1:1004983/1‑62 (MQ=255) cGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTg > 1:663757/1‑62 (MQ=255) cGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTg > 1:497385/1‑62 (MQ=255) cGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTg > 1:1424505/1‑62 (MQ=255) cGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTg > 1:2144269/1‑62 (MQ=255) cGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTg > 1:2854005/1‑62 (MQ=255) cGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTg > 1:1759457/1‑62 (MQ=255) | CGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTG > W3110S.gb/4489095‑4489191 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |