Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,583,739	A→G	F94S (TTC→TCC)	mcrB ←	5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,583,739	0	A	G	100.0%	98.4 / NA	30	F94S (TTC→TCC)	mcrB	5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/10);  total (20/10)

GAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGAACTGCCATTGGGCATGTGGTTCATTCG  >  W3110S.gb/4583691‑4583766                                                 |                            gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTggg                 >  1:1957779/1‑61 (MQ=255) gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTggg                 >  1:2391446/1‑61 (MQ=255) gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTggg                 >  1:2802980/1‑61 (MQ=255) gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc                >  1:974897/1‑62 (MQ=255) gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc                >  1:1068118/1‑62 (MQ=255) gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc                >  1:785035/1‑62 (MQ=255) gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc                >  1:761710/1‑62 (MQ=255) gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc                >  1:666816/1‑62 (MQ=255) gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc                >  1:663317/1‑62 (MQ=255) gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc                >  1:618895/1‑62 (MQ=255) gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc                >  1:55845/1‑62 (MQ=255) gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc                >  1:483033/1‑62 (MQ=255) gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc                >  1:297154/1‑62 (MQ=255) gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc                >  1:2901177/1‑62 (MQ=255) gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc                >  1:2473939/1‑62 (MQ=255) gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc                >  1:2370124/1‑62 (MQ=255) gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc                >  1:2224956/1‑62 (MQ=255) gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc                >  1:2165872/1‑62 (MQ=255) gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc                >  1:203838/1‑62 (MQ=255) gAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGc                >  1:174301/1‑62 (MQ=255)               aTACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGCATGTGGTTCATTCg  <  1:2696615/62‑1 (MQ=255)               aTACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGCATGTGGTTCATTCg  <  1:2913344/62‑1 (MQ=255)               aTACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGCATGTGGTTCATTCg  <  1:2319776/62‑1 (MQ=255)               aTACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGCATGTGGTTCATTCg  <  1:1671930/62‑1 (MQ=255)                tACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGCATGTGGTTCATTCg  <  1:2537558/61‑1 (MQ=255)                tACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGCATGTGGTTCATTCg  <  1:2817502/61‑1 (MQ=255)                tACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGCATGTGGTTCATTCg  <  1:2197808/61‑1 (MQ=255)                tACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGCATGTGGTTCATTCg  <  1:956923/61‑1 (MQ=255)                tACTCTGCGATTGTTTTAGGTATGTCTGAAGAGGACTGCCATTGGGCATGTGGTTCATTCg  <  1:1163348/61‑1 (MQ=255)                                       gTCTGAAGAGGACTGCCATTGGGCATGTGGTTCATTCg  <  1:2835546/38‑1 (MQ=255)                                                 |                            GAAGTTGCCTGAAAATACTCTGCGATTGTTTTAGGTATGTCTGAAGAGAACTGCCATTGGGCATGTGGTTCATTCG  >  W3110S.gb/4583691‑4583766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑