Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	266,076	A→G	V39A (GTG→GCG)	yafY ←	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	266,076	0	A	G	100.0%	325.2 / NA	95	V39A (GTG→GCG)	yafY	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/56);  total (39/56)

ATCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACACACCAAACTCAGCGGCAAGCTTACGCACACTCA  >  W3110S.gb/266034‑266109                                           |                                  aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCaa                 >  1:140857/1‑61 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCa                  >  1:1680039/1‑60 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:2026958/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:559411/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:1807062/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:1823853/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:545583/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:2447544/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:2427419/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:2360271/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:492212/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:2580368/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:234029/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:2032087/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:2115526/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:2127835/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:423521/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:33664/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:382352/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:364316/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:2290275/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:2327236/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:621982/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:1039796/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:1169504/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:2579866/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:858418/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:1239774/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:1307018/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:789225/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:1410653/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:704937/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:2532977/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:2736994/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:150799/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:156119/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAg                >  1:1544410/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGACAAg                >  1:1785650/1‑62 (MQ=255) aTCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCAACGCACCAAACTCAGCGGCaa                 >  1:1069986/1‑61 (MQ=255)  tCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGc               <  1:2500577/62‑1 (MQ=255)  tCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGc               <  1:1466891/62‑1 (MQ=255)  tCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGc               <  1:1091257/62‑1 (MQ=255)  tCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGc               <  1:2169400/62‑1 (MQ=255)  tCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGc               <  1:1689353/62‑1 (MQ=255)  tCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGc               <  1:1704682/62‑1 (MQ=255)  tCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGc               <  1:2078815/62‑1 (MQ=255)  tCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGc               <  1:821923/62‑1 (MQ=255)  tCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGc               <  1:1726294/62‑1 (MQ=255)  tCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGc               <  1:773615/62‑1 (MQ=255)  tCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGc               <  1:1978438/62‑1 (MQ=255)            aCGAAAATCACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGc               <  1:372690/52‑1 (MQ=255)                    cACGCCGCAGCGTTCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:1978929/57‑1 (MQ=25)                    cACGCCGCAGCGTGCTCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:1859705/57‑1 (MQ=25)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:417849/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:443277/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:507722/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:333297/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:511278/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:306585/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:967089/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:2776719/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:580750/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:68116/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:702325/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:886842/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:1008266/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:2530757/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:1032290/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:1224824/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:1237549/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:1327151/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:1333997/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:142776/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:1462154/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:1495693/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:158453/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:1618592/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:1722162/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:183684/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:1894335/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:2015241/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:2027443/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:2138526/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:2155503/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:2246078/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:2247977/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:2280484/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:2332636/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:2398359/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:2438302/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:2456390/57‑1 (MQ=255)                    cACGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:2710205/57‑1 (MQ=255)                     aCGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:1948810/56‑1 (MQ=255)                     aCGCCGCAGCGTGCGCACCGACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:1253510/56‑1 (MQ=255)                                        gACGCACCAAACTCAGCGGCAAGCTTACGCACACTCa  <  1:505395/37‑1 (MQ=255)                                           |                                  ATCAGCCGCTCACGAAAATCACGCCGCAGCGTGCGCACCGACACACCAAACTCAGCGGCAAGCTTACGCACACTCA  >  W3110S.gb/266034‑266109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑