Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,632,877 A→G Y294C (TAC→TGC)  nadR → bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,632,8770AG100.0% 109.4 / NA 33Y294C (TAC→TGC) nadRbifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/1);  total (32/1)

TCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATC  >  W3110S.gb/4632826‑4632920
                                                   |                                           
tCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGAtttt                                   >  1:811488/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:1195347/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:686288/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:652508/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:640171/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:479534/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:315549/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:301270/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:300202/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:2885247/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:2866237/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:2693400/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:2610180/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:2466/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:2428962/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:2352875/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:1070584/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:140562/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:1539062/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:1593702/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:1676781/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:176205/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:1820684/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:2020977/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:2335182/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:2392095/1‑62 (MQ=255)
                    tACGATAAAATCGCGCTGGGACACGCTCAATGCATTGATTTTGCGGTGAAata                        >  1:2737033/1‑53 (MQ=255)
                    tACGATAAAATCGAGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATaa               >  1:1313439/1‑62 (MQ=255)
                          aaaaTCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGc         <  1:762825/62‑1 (MQ=255)
                                  gcTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATc  >  1:184954/1‑61 (MQ=255)
                                  gcTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATc  >  1:2294910/1‑61 (MQ=255)
                                  gcTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATc  >  1:2591847/1‑61 (MQ=255)
                                  gcTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATc  >  1:90461/1‑61 (MQ=255)
                                                   |                                           
TCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATC  >  W3110S.gb/4632826‑4632920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: