Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	300,837	G→A	S92F (TCC→TTC)	yagS ←	predicted oxidoreductase with FAD‑binding domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	300,837	0	G	A	100.0%	160.5 / NA	49	S92F (TCC→TTC)	yagS	predicted oxidoreductase with FAD‑binding domain
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (49/0);  total (49/0)

GGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCA  >  W3110S.gb/300785‑300897                                                     |                                                             ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGaag                                                            >  1:1345709/1‑55 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:2831410/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:2030622/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:2165370/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:2176175/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:2279803/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:2365554/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:2365657/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:2435113/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:2576199/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:2593282/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:2619804/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:2621077/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:2627293/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:2629623/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:2674477/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:2738817/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:1058772/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:2857515/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:325961/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:340708/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:445800/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:563347/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:567690/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:597894/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:685924/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:713078/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:7496/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:91424/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:982158/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:994555/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:1396264/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:1000501/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:1076389/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:1096613/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:110599/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:1150412/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:1185248/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:1334378/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:1394807/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:1695944/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:1783191/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:1861154/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:1974727/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAcac                                                     >  1:1916407/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAca                                                      >  1:1062952/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAca                                                      >  1:2358324/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAca                                                      >  1:249176/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAca                                                      >  1:2204752/1‑60 (MQ=255) ggtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGAAGAGCAca                                                      >  1:2730384/1‑60 (MQ=255)                                                    ggAGAGTACCGCGTATTCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:1205970/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGTACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:602496/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:1127127/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:528971/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:565697/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:2196319/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:616670/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:650342/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:1250532/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:1957120/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:729918/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:786520/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:876934/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:2055361/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:952992/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:2043686/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:1619542/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:2294898/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:18302/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:2289011/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:2456097/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:2572133/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:1766273/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:2286167/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:1655252/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:1225399/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:1486083/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:1401085/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:2673581/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:2018634/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:1362184/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:2232993/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:1332451/62‑1 (MQ=255)                                                    ggAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa  <  1:436652/62‑1 (MQ=255)                                                     |                                                             GGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCA  >  W3110S.gb/300785‑300897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑