Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	360,737	A→C	P116P (CCT→CCG)	lacA ←	thiogalactoside acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	360,737	0	A	C	94.7%	57.4 / ‑4.0	19	P116P (CCT→CCG)	lacA	thiogalactoside acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (0/18);  total (0/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATCGGAAAAGAGTACATCTCGCCGTTTTTTCTCAATTCATGGTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGT  >  W3110S.gb/360689‑360765                                                 |                             tATCGGAAAAGAGTACATCTCTCCGTTTTTTCTCAATTCATGGTGTACCGGGTGTCCCg                    <  1:2332924/59‑1 (MQ=255) tATCGGAAAAGAGTACATCTCTCCGTTTTTTCTCAATTCATGGTGTACCGGGTGTCCCg                    <  1:631517/59‑1 (MQ=255) tATCGGAAAAGAGTACATCTCTCCGTTTTTTCTCAATTCATGGTGTACCGGGTGTCCCg                    <  1:619274/59‑1 (MQ=255) tATCGGAAAAGAGTACATCTCTCCGTTTTTTCTCAATTCATGGTGTACCGGGTGTCCCg                    <  1:395434/59‑1 (MQ=255) tATCGGAAAAGAGTACATCTCTCCGTTTTTTCTCAATTCATGGTGTACCGGGTGTCCCg                    <  1:2900786/59‑1 (MQ=255) tATCGGAAAAGAGTACATCTCTCCGTTTTTTCTCAATTCATGGTGTACCGGGTGTCCCg                    <  1:287885/59‑1 (MQ=255) tATCGGAAAAGAGTACATCTCTCCGTTTTTTCTCAATTCATGGTGTACCGGGTGTCCCg                    <  1:2801804/59‑1 (MQ=255) tATCGGAAAAGAGTACATCTCTCCGTTTTTTCTCAATTCATGGTGTACCGGGTGTCCCg                    <  1:2447114/59‑1 (MQ=255) tATCGGAAAAGAGTACATCTCTCCGTTTTTTCTCAATTCATGGTGTACCGGGTGTCCCg                    <  1:2438504/59‑1 (MQ=255) tATCGGAAAAGAGTACATCTCTCCGTTTTTTCTCAATTCATGGTGTACCGGGTGTCCCg                    <  1:1026478/59‑1 (MQ=255) tATCGGAAAAGAGTACATCTCTCCGTTTTTTCTCAATTCATGGTGTACCGGGTGTCCCg                    <  1:2293337/59‑1 (MQ=255) tATCGGAAAAGAGTACATCTCTCCGTTTTTTCTCAATTCATGGTGTACCGGGTGTCCCg                    <  1:2112635/59‑1 (MQ=255) tATCGGAAAAGAGTACATCTCTCCGTTTTTTCTCAATTCATGGTGTACCGGGTGTCCCg                    <  1:2031023/59‑1 (MQ=255) tATCGGAAAAGAGTACATCTCTCCGTTTTTTCTCAATTCATGGTGTACCGGGTGTCCCg                    <  1:1277089/59‑1 (MQ=255) tATCGGAAAAGAGTACATCTCTCCGTTTTTTCTCAATTCATGGTGTACCGGGTGTCCCg                    <  1:1255202/59‑1 (MQ=255) tATCGGAAAAGAGTACATCTCTCCGTTTTTTCTCAATTCATGGTGTACCGGGTGTCCCg                    <  1:1230260/59‑1 (MQ=255) tATCGGAAAAGAGTACATCTCTCCGTTTTTTCTCAATTCATGGTGTACCGGGTGTCCCg                    <  1:109671/59‑1 (MQ=255) tATCGGAAAAGAGTACATCTCTCCGTTTTTTCTCAATTCATGGTGTACCGGGTGTCCCg                    <  1:1032843/59‑1 (MQ=255)                cATCTCGCCGTTTTTTCTCAATTCATGGTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGt  <  1:2494869/62‑1 (MQ=255)                                                 |                             TATCGGAAAAGAGTACATCTCGCCGTTTTTTCTCAATTCATGGTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGT  >  W3110S.gb/360689‑360765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑