| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 361,710 | C→T | V232I (GTT→ATT) | lacY ← | lactose/galactose transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 361,710 | 0 | C | T | 100.0% | 13.7 / NA | 6 | V232I (GTT→ATT) | lacY | lactose/galactose transporter |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (0/6); total (0/6) | |||||||||||
AAACATCGTAGGTGCAGGAAACGCCAATAACATACAGTGACAAAAACCACAGTTTTGGCTG > W3110S.gb/361689‑361749 | aaaCATCGTAGGTGCAGGAAATGCCAATAACATACAGTGACAAAAACCACAGTTTTGGCTg < 1:1104083/61‑1 (MQ=255)aaaCATCGTAGGTGCAGGAAATGCCAATAACATACAGTGACAAAAACCACAGTTTTGGCTg < 1:582740/61‑1 (MQ=255)aaaCATCGTAGGTGCAGGAAATGCCAATAACATACAGTGACAAAAACCACAGTTTTGGCTg < 1:599935/61‑1 (MQ=255)aaaCATCGTAGGTGCAGGAAATGCCAATAACATACAGTGACAAAAACCACAGTTTTGGCTg < 1:693855/61‑1 (MQ=255)aaaCATCGTAGGTGCAGGAAATGCCAATAACATACAGTGACAAAAACCACAGTTTTGGCTg < 1:697093/61‑1 (MQ=255) aaCATCGTAGGTGCAGGAAATGCCAATAACATACAGTGACAAAAACCACAGTTTTGGCTg < 1:2251712/60‑1 (MQ=255) | AAACATCGTAGGTGCAGGAAACGCCAATAACATACAGTGACAAAAACCACAGTTTTGGCTG > W3110S.gb/361689‑361749 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |