Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 406,678 C→T T9T (ACC→ACT aroM → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb406,6780CT98.6% 239.5 / ‑4.6 73T9T (ACC→ACTaroMconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (72/0);  total (73/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

TGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGACGGAATACATTGA  >  W3110S.gb/406665‑406731
             |                                                     
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2774594/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:368725/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:35738/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2926710/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2923382/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2922057/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2921973/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2913273/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2843449/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:377851/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2655830/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2627192/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2602786/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2593280/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2568869/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2449442/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2449433/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2421890/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:671991/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:986744/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:953219/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:952366/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:776269/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:743071/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:708692/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:693883/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:675442/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2367973/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:591278/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:562479/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:524215/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:497050/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:475655/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:44103/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:417942/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:414234/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:1570880/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:1861447/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:185702/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:184471/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:1818026/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:1730996/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:1705612/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:1701988/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:1682729/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:1874931/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:156761/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:1567410/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:1553201/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:1459865/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:1317199/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:1283757/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:1208722/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:1128346/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2202998/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2360118/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2326087/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2313786/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2245109/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2220829/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2212380/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2209223/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:1041058/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2157798/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:21321/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2088457/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:2010052/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:1982274/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:1931430/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:1913360/1‑35 (MQ=255)
tGGCGATCCTGACTATCGGCATTGTACCTATGCAg                                  >  1:1877183/1‑35 (MQ=255)
tGGCGATCATGACTATCGGCATTGTACCTATGCAg                                  >  1:2443917/1‑35 (MQ=25)
        cTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGACGGAATACATTGa  >  1:1511189/1‑59 (MQ=255)
             |                                                     
TGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGACGGAATACATTGA  >  W3110S.gb/406665‑406731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: