| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 429,759 | T→A | intergenic (+59/‑70) | secF → / → yajD | SecYEG protein translocase auxillary subunit/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 429,759 | 0 | T | A | 81.6% | 104.8 / 19.6 | 49 | intergenic (+59/‑70) | secF/yajD | SecYEG protein translocase auxillary subunit/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (7/2); new base A (0/40); total (7/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.19e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.99e-01 | |||||||||||
TCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTC > W3110S.gb/429707‑429812 | tCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTg > 1:2348104/1‑61 (MQ=255)tCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTg > 1:527544/1‑61 (MQ=255)tCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTg > 1:525627/1‑61 (MQ=255)tCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTg > 1:2134999/1‑61 (MQ=255)tCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTg > 1:1731472/1‑61 (MQ=255)tCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTg > 1:2072952/1‑61 (MQ=255)tCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTg > 1:1746963/1‑61 (MQ=255) aTTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCt < 1:133079/62‑1 (MQ=255) aTTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCt < 1:1635191/62‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:387628/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2321770/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2429130/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2529183/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2607741/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2674720/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2774684/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2781401/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2787180/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2804222/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2244557/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:444440/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:481940/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:523433/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:525994/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:681927/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:914108/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2286030/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:111645/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:1188332/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:1212526/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:1331440/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:1514773/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:1618053/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:1657256/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:1736233/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:1748739/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:177620/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:1794408/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:1808801/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:1883844/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2006540/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2035986/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2107522/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2143410/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:1094580/61‑1 (MQ=255) tGATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2319410/61‑1 (MQ=255) gATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:2911600/60‑1 (MQ=255) gATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:496643/60‑1 (MQ=255) gATGTAAGGTGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc < 1:824696/60‑1 (MQ=255) | TCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTC > W3110S.gb/429707‑429812 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |