Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	520,140	C→T	P167P (CCC→CCT)	ybbP →	predicted inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	520,140	0	C	T	100.0%	30.8 / NA	11	P167P (CCC→CCT)	ybbP	predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/11);  total (0/11)

TTTAACCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACC  >  W3110S.gb/520132‑520191         |                                                    tttAATCCTTTCCAGATAGCCCCACGCCTGATGATGAATCTGGCGGATGTTGATAAAAcc  <  1:1081354/60‑1 (MQ=255) tttAATCCTTTCCAGATAGCCCCACGCCTGATGATGAATCTGGCGGATGTTGATAAAAcc  <  1:1315447/60‑1 (MQ=255) tttAATCCTTTCCAGATAGCCCCACGCCTGATGATGAATCTGGCGGATGTTGATAAAAcc  <  1:1563257/60‑1 (MQ=255) tttAATCCTTTCCAGATAGCCCCACGCCTGATGATGAATCTGGCGGATGTTGATAAAAcc  <  1:1773794/60‑1 (MQ=255) tttAATCCTTTCCAGATAGCCCCACGCCTGATGATGAATCTGGCGGATGTTGATAAAAcc  <  1:2222763/60‑1 (MQ=255) tttAATCCTTTCCAGATAGCCCCACGCCTGATGATGAATCTGGCGGATGTTGATAAAAcc  <  1:2373303/60‑1 (MQ=255) tttAATCCTTTCCAGATAGCCCCACGCCTGATGATGAATCTGGCGGATGTTGATAAAAcc  <  1:2467279/60‑1 (MQ=255) tttAATCCTTTCCAGATAGCCCCACGCCTGATGATGAATCTGGCGGATGTTGATAAAAcc  <  1:2563174/60‑1 (MQ=255) tttAATCCTTTCCAGATAGCCCCACGCCTGATGATGAATCTGGCGGATGTTGATAAAAcc  <  1:2703232/60‑1 (MQ=255) tttAATCCTTTCCAGATAGCCCCACGCCTGATGATGAATCTGGCGGATGTTGATAAAAcc  <  1:2705402/60‑1 (MQ=255) tttAATCCTTTCCAGATAGCCCCACGCCTGATGATGAATCTGGCGGATGTTGATAAAAcc  <  1:910882/60‑1 (MQ=255)         |                                                    TTTAACCCCTTCCAGATGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACC  >  W3110S.gb/520132‑520191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑