Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	47,533	T→C	H96H (CAT→CAC)	kefF →	flavoprotein subunit for the KefC potassium efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	47,533	0	T	C	100.0%	200.2 / NA	59	H96H (CAT→CAC)	kefF	flavoprotein subunit for the KefC potassium efflux system
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/59);  total (0/59)

GCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCATTT  >  W3110S.gb/47527‑47583       |                                                   gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCTGGGAAAGCCAttt  <  1:558662/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:485297/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:2193351/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:2219338/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:2344330/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:2372420/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:2405845/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:2413031/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:2456955/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:2609580/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:2664729/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:2672735/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:2756816/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:2841298/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:386410/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:4260/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:2175513/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:507213/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:592661/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:644096/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:676788/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:705098/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:729040/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:755616/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:832532/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:853111/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:921695/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:96091/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:990043/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:1579303/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:1010600/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:1084694/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:1140027/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:1247150/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:1270139/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:1272329/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:1281563/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:1286780/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:1306476/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:1316486/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:1327083/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:1452018/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:1513237/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:1009587/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:1804183/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:1811155/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:1921946/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:1933673/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:194354/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:1975467/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:1996416/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:2073934/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:2123599/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:2147525/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:2162066/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:2163343/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:2169543/57‑1 (MQ=255) gcTGCACGGCAAACATTTGCTGTCGGCGGTGACGACCGGCGGCGGGGAAAGCCAttt  <  1:2766307/57‑1 (MQ=255)  cTGCACGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCTGGGAAAGCCAttt  <  1:1276642/56‑1 (MQ=255)       |                                                   GCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCATTT  >  W3110S.gb/47527‑47583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑