| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 616,379 | C→T | F1000F (TTC→TTT) | entF → | enterobactin synthase multienzyme complex component, ATP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 616,379 | 0 | C | T | 95.6% | 208.9 / ‑0.4 | 68 | F1000F (TTC→TTT) | entF | enterobactin synthase multienzyme complex component, ATP‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (3/0); new base T (0/65); total (3/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 161 total aligned reads displayed.
TCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAG > W3110S.gb/616318‑616436 | tCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATttcttc > 1:2397124/1‑62 (MQ=255)tCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATttcttc > 1:979703/1‑62 (MQ=255)tCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATttcttc > 1:767014/1‑62 (MQ=255)tCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATttcttc > 1:69842/1‑62 (MQ=255)tCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATttcttc > 1:365076/1‑62 (MQ=255)tCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATttcttc > 1:316399/1‑62 (MQ=255)tCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATttcttc > 1:308709/1‑62 (MQ=255)tCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATttcttc > 1:2671051/1‑62 (MQ=255)tCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATttcttc > 1:256233/1‑62 (MQ=255)tCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATttcttc > 1:1990204/1‑62 (MQ=255)tCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATttcttc > 1:1945600/1‑62 (MQ=255)tCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATttcttc > 1:1925356/1‑62 (MQ=255)tCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATttcttc > 1:1626788/1‑62 (MQ=255)tCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATttcttc > 1:1592711/1‑62 (MQ=255)tCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATttcttc > 1:1119278/1‑62 (MQ=255)tCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATttcttc > 1:122696/1‑62 (MQ=255) tgtgACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCaa > 1:1461658/1‑61 (MQ=255) tgtgACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCaa > 1:2728867/1‑61 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGGAACTGGCAGCGCAGTTa < 1:409819/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:511371/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:2132662/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:2172076/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:2342954/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:1066164/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:2072492/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:2436324/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:804358/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:772481/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:1176718/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:1191317/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:2640177/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:266220/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:65792/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:2810328/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:612875/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:2859983/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:2983/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:1264974/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:327448/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:391957/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:520001/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:42814/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:436725/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:481121/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:203333/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:1476360/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:8973/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:1382935/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:1744207/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:1855866/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:1879960/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:875245/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:1897207/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:1372007/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:836508/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:1371089/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:2057593/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:2028709/62‑1 (MQ=255) aTGCTGATTTCTTTGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:2002133/62‑1 (MQ=255) aTGCTGATTTCTTTGCCCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:1426750/62‑1 (MQ=255) cTGATTTCTTTGCGATTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTa < 1:2811436/59‑1 (MQ=255) ttctttGCGCTTGGCGGTCATTCGCTACTGGAAATTAAACTGGCAGCGCAGTTa < 1:1349856/54‑1 (MQ=255) tcttcGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTcagcag > 1:1650473/1‑62 (MQ=255) tcttcGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAg > 1:1540645/1‑62 (MQ=255) tcttcGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAg > 1:147803/1‑62 (MQ=255) tcttcGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAg > 1:1388756/1‑62 (MQ=255) tcttcGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAg > 1:1613630/1‑62 (MQ=255) tcttcGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAg > 1:566421/1‑62 (MQ=255) tcttcGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAg > 1:1662325/1‑62 (MQ=255) tcttcGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAg > 1:1722697/1‑62 (MQ=255) tcttcGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAg > 1:293400/1‑62 (MQ=255) tcttcGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAg > 1:2911631/1‑62 (MQ=255) tcttcGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAg > 1:2249883/1‑62 (MQ=255) tcttcGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAg > 1:51028/1‑62 (MQ=255) tcttcGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAg > 1:1865749/1‑62 (MQ=255) tcttcGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAg > 1:2732819/1‑62 (MQ=255) tcttcGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAg > 1:2704136/1‑62 (MQ=255) tcttcGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAg > 1:1291142/1‑62 (MQ=255) tcttcGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAg > 1:836296/1‑62 (MQ=255) cttcGCGCTTGTCGGTCATTCGCTACTGGCAATGa > 1:1902172/1‑35 (MQ=255) cttcGCGCTTGGCGGTCATTCGCTACTGGCAATGa > 1:807709/1‑35 (MQ=255) cttcGCGCTTGGCGGTCATTCGCTACTGGCAATGa > 1:541158/1‑35 (MQ=255) cttcGCGCTTGGCGGTCATTCGCTACTGGCAATGa > 1:894944/1‑35 (MQ=255) cttcGCGCTTGGCGGTCATTCGCTACTGGCAATGa > 1:2654547/1‑35 (MQ=255) cttcGCGCTTGGCGGTCATTCGCTACTGGCAATGa > 1:402836/1‑35 (MQ=255) cttcGCGCTTGGCGGTCATTCGCTACTGGCAATGa > 1:369931/1‑35 (MQ=255) cttcGCGCTTGGCGGTCATTCGCTACTGGCAATGa > 1:1256129/1‑35 (MQ=255) cttcGCGCTTGGCGGTCATTCGCTACTGGCAATGa > 1:2857168/1‑35 (MQ=255) cttcGCGCTTGGCGGTCATTCGCTACTGGCAATGa > 1:2782919/1‑35 (MQ=255) cttcGCGCTTGGCGGTCATTCGCTACTGGCAATGa > 1:2595642/1‑35 (MQ=255) cttcGCGCTTGGCGGTCATTCGCTACTGGCAATGa > 1:131521/1‑35 (MQ=255) cttcGCGCTTGGCGGTCATTCGCTACTGGCAATGa > 1:2553449/1‑35 (MQ=255) cttcGCGCTTGGCGGTCATTCGCTACTGGCAATGa > 1:2509282/1‑35 (MQ=255) cttcGCGCTTGGCGGTCATTCGCTACTGGCAATGa > 1:2146768/1‑35 (MQ=255) cttcGCGCTTGGCGGTCATTCGCTACTGGCAATGa > 1:1930582/1‑35 (MQ=255) cttcGCGCTTGGCGGTCATTCGCTACTGGCAATGa > 1:1927425/1‑35 (MQ=255) cttcGCGCTTGGCGGTCATTCGCTACTGGCAATGa > 1:1883654/1‑35 (MQ=255) cttcGCGCTTGGCGGTCATTCGCTACTGGCAATGa > 1:1852677/1‑35 (MQ=255) cttcGCGCTTGGCGGTCATTCGCTACTGGCAATGa > 1:1579521/1‑35 (MQ=255) cttcGCGCTTGGCGGTCATTCGCTACTGGCAATGa > 1:1459669/1‑35 (MQ=255) | TCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAG > W3110S.gb/616318‑616436 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |