Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	624,578	C→T	T157T (ACC→ACT)	entC →	isochorismate synthase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	624,578	0	C	T	100.0%	108.1 / NA	33	T157T (ACC→ACT)	entC	isochorismate synthase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/0);  total (33/0)

ATCACCACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAACCC  >  W3110S.gb/624573‑624634      |                                                         atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:2538365/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:995590/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:903327/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:893055/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:839544/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:826343/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:652147/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:391702/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:318919/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:284089/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:2816903/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:2783190/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:2705076/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:2702342/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:2675096/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:2552581/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:2058494/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:1360972/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:1569209/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:166541/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:1807566/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:1842908/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:1885603/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:2044116/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:1040217/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:2144255/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:2161603/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:2171631/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:2193604/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:2243662/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:2370170/1‑62 (MQ=255) atCACTACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGAATGCGCAAAAccc  >  1:811564/1‑62 (MQ=255) atCACTAATGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAAccc  >  1:2101284/1‑62 (MQ=255)      |                                                         ATCACCACTGACGCCGCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAACCC  >  W3110S.gb/624573‑624634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑