Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	687,819	G→A	N117N (AAC→AAT)	gltI ←	glutamate and aspartate transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	687,819	0	G	A	100.0%	56.5 / NA	18	N117N (AAC→AAT)	gltI	glutamate and aspartate transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/18);  total (0/18)

GCCTGTTTTTGGCGTTCGACGTTGTTGGTGGTAGAACCACATTCAAAATCGAAAGTGCCGTT  >  W3110S.gb/687799‑687860                     |                                          gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt  <  1:2014438/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt  <  1:853765/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt  <  1:784197/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt  <  1:723775/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt  <  1:41260/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt  <  1:2326234/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt  <  1:2321263/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt  <  1:2269656/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt  <  1:2087146/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt  <  1:1283474/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt  <  1:184759/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt  <  1:1839872/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt  <  1:1759828/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt  <  1:1732857/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt  <  1:1650437/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt  <  1:1445639/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt  <  1:1382584/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt  <  1:1374942/62‑1 (MQ=255)                     |                                          GCCTGTTTTTGGCGTTCGACGTTGTTGGTGGTAGAACCACATTCAAAATCGAAAGTGCCGTT  >  W3110S.gb/687799‑687860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑