Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	687,837	A→G	C111C (TGT→TGC)	gltI ←	glutamate and aspartate transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	687,837	0	A	G	86.4%	49.5 / 1.3	22	C111C (TGT→TGC)	gltI	glutamate and aspartate transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/3);  new base G (0/19);  total (0/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

GCCTGTTTTTGGCGTTCGACGTTGTTGGTGGTAGAACCACATTCAAAATCGAAAGTGCCGTTTTGCAGCAGTGGAATACGGTTTTG  >  W3110S.gb/687799‑687884                                       |                                                gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt                          <  1:2014438/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt                          <  1:853765/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt                          <  1:784197/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt                          <  1:723775/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt                          <  1:41260/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt                          <  1:2326234/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt                          <  1:2321263/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt                          <  1:2269656/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt                          <  1:2087146/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt                          <  1:184759/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt                          <  1:1839872/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt                          <  1:1759828/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt                          <  1:1732857/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt                          <  1:1650437/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt                          <  1:1445639/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt                          <  1:1382584/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt                          <  1:1374942/62‑1 (MQ=255) gccTGTTTTTGGCGTTCGGCATTGTTGGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt                          <  1:1283474/62‑1 (MQ=255)                     attgttgGTGGTAGAACCGCATTCAAAATCGAAAGTGCCGtt                          <  1:57474/41‑1 (MQ=39)                          tggtggTAGAACCACATTCAAAATCGAAAGTGCCGTTTTGCAGCAGTGGAATACGGTTTtg  <  1:1279849/61‑1 (MQ=255)                          tggtggTAGAACCACATTCAAAATCGAAAGTGCCGTTTTGCAGCAGTGGAATACGGTTTtg  <  1:1744058/61‑1 (MQ=255)                          tggtggTAGAACCACATTCAAAATCGAAAGTGCCGTTTTGCAGCAGTGGAATACGGTTTtg  <  1:2376017/61‑1 (MQ=255)                                       |                                                GCCTGTTTTTGGCGTTCGACGTTGTTGGTGGTAGAACCACATTCAAAATCGAAAGTGCCGTTTTGCAGCAGTGGAATACGGTTTTG  >  W3110S.gb/687799‑687884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑