Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	698,544	A→G	D352D (GAT→GAC)	asnB ←	asparagine synthetase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	698,544	0	A	G	81.2%	170.3 / 30.2	80	D352D (GAT→GAC)	asnB	asparagine synthetase B
Reads supporting (aligned to +/- strand):  ref base A (8/7);  new base G (65/0);  total (73/7)
Fisher's exact test for biased strand distribution p-value = 2.03e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAACCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACA  >  W3110S.gb/698492‑698602                                                     |                                                           actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATACCCGCCAAACACTTCGTCAGAAcc                                                    >  1:1545580/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:262592/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:2881302/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:2809079/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:2770956/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:2687232/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:2685130/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:2668300/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:266222/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:2904944/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:2625374/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:2579268/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:2525486/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:2512117/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:1050263/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:2206554/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:2172565/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:2151198/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:613337/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:991186/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:875215/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:850708/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:815271/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:780221/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:745453/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:688528/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:685202/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:2314556/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:560174/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:518917/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:488493/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:428210/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:413028/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:37198/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:360660/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:341729/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:1279343/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:1538298/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:1537512/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:1493428/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:1458504/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:1424040/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:138527/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:1365653/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:1307368/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:211495/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:1252955/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:1237735/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:123421/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:1163232/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:1148757/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:1144755/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:1098349/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:1710346/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:1985021/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:2006803/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:2069984/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:1858945/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:1795040/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:1776271/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:1560533/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:1686323/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:1617689/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:1607720/3‑61 (MQ=255) actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc                                                    >  1:1587560/3‑61 (MQ=255)                     ttGTGGAAGTAAAGATAACCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCAtttt                               >  1:485909/1‑62 (MQ=255)                     ttGTGGAAGTAAAGATAACCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCAtttt                               >  1:1731010/1‑62 (MQ=255)                     ttGTGGAAGTAAAGATAACCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCAtttt                               >  1:1764157/1‑62 (MQ=255)                     ttGTGGAAGTAAAGATAACCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCAtttt                               >  1:1529149/1‑62 (MQ=255)                     ttGTGGAAGTAAAGATAACCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCAtttt                               >  1:2781008/1‑62 (MQ=255)                     ttGTGGAAGTAAAGATAACCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCAtttt                               >  1:2824189/1‑62 (MQ=255)                     ttGTGGAAGTAAAGATAACCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCAtttt                               >  1:2367168/1‑62 (MQ=255)                     ttGTGGAAGTAAAGATAACCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCAttt                                >  1:680163/1‑61 (MQ=255)                                                  ttCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACa  <  1:565042/62‑1 (MQ=255)                                                  ttCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACa  <  1:2132311/62‑1 (MQ=255)                                                  ttCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACa  <  1:1093037/62‑1 (MQ=255)                                                  ttCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACa  <  1:749506/62‑1 (MQ=255)                                                  ttCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACa  <  1:723948/62‑1 (MQ=255)                                                  ttCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACa  <  1:2817672/62‑1 (MQ=255)                                                  ttCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCCTGATCTTACGCGACa  <  1:723112/62‑1 (MQ=255)                                                   tcatcaGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACa  <  1:1552178/61‑1 (MQ=255)                                                     |                                                           GTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAACCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACA  >  W3110S.gb/698492‑698602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑